DNF-473-0500

Small Fragment and NGS Kits

RUO

The Agilent NGS and Small Fragment kits simplify NGS library quality control (QC) and analysis of DNA smears and fragments. These kits provide accurate quantification and sizing, saving you time during NGS workflows.

The Small Fragment kits cover a sizing range of 50 to 1,500 bp, making them ideal for the analysis of small fragments, cfDNA, and small RNA library sample QC. The NGS Fragment kits have a sizing range that is extended to 6,000 bp and focus on NGS library QC. The Small Fragment and NGS Fragment kits are specific for high input concentration ranges, while the HS Small Fragment and HS NGS Fragment kits are designed for a lower concentration range.

For Research Use Only. Not for use in diagnostic procedures.

Fragment Analyzer Systems DNA Analysis Kits

Product Details

Features

High and standard sensitivity – Detect DNA fragments in the concentration range of 0.1 to 10 ng/µL with the NGS and Small Fragment kits, while the HS NGS and HS Small Fragment kits allow a lower concentration range of 5 to 500 pg/µL
Wide smear concentration range – Analyze smears in the concentration range of 5 to 100 ng/µL with the NGS and Small Fragment kits, and a lower concentration sample of 50 to 5,000 pg/µL with the HS NGS and HS Small Fragment kits
Reliable sizing – Provide accurate and precise sizing for wide size range of DNA fragments and smears, covering 50 to 1,500 bp with the Small Fragment kits and 100 to 6,000 bp with the NGS kits
Specific sizing ranges - The Small Fragment kits have a sizing range of 50 to 1,500 bp, while the NGS kits cover 100 to 6,000 bp, ensuring accurate sizing for specific applications
Low sample volume – Require only 2 µL of sample, minimizing sample loss to QC steps

Specifications


Guaranteed Shelf Life	Minimum 4 months
Kit Type	Quantitative

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How It Works

Quality control steps to determine presence of adapter dimers in NGS libraries

With the increasing sensitivity of sequencing platforms, it is crucial to adopt measures that guarantee sequencing libraries possess minimal levels of undesired products, which could potentially hinder the accuracy of sequencing outcomes. Using the HS NGS or HS Small Fragment kits, the Agilent Fragment Analyzer systems provide precise evaluations regarding the proportion of adapter dimers present in NGS libraries. This information aids researchers in making critical decisions for their sequencing workflows.

Read how to detect adapter dimers

Cell-free DNA analysis with the HS Small Fragment kit

Quality analysis plays an important role in determining sizing and purity of extracted, circulating cell-free DNA (cfDNA). The Fragment Analyzer systems offer excellent resolution and sizing of the first three nucleosome peaks with the HS Small Fragment kit. This kit provides efficient and excellent separations of cfDNA extracted with or without carrier RNA.

Learn more about cfDNA analysis

Best sizing practices with the Small Fragment and NGS kits

QC checkpoints for sizing are a necessity for many nucleic acid applications and ensure successful outcomes. The Fragment Analyzer systems combined with the NGS Fragment, HS NGS Fragment, Small Fragment, and HS Small Fragment kits provide reliable sizing for fragments and smears from 50 to 6,000 bp.

Learn more about best sizing practices

Literature

Key Literature

Detection of Adapter Dimers in NGS Libraries with the Agilent Fragment analyzer and TapeStation Systems

This technical overview highlights the sensitivity and resolution of the Agilent Fragment Analyzer and TapeStation systems to detect adapter dimers lower than the recommended threshold of 0.5%.

Technical Overviews
English
06 Jun 2023
1.45 MB
PDF

Build Consistency into Your Lab’s Workflow with the Agilent Automated Electrophoresis Portfolio.

The automated electrophoresis instrument portfolio includes systems for objective assessment of sample integrity, concentration, and fragment size. Download this eBrochure for an interactive experience.

Brochures
English
14 May 2024
12.33 MB
PDF

Nucleic Acid Analysis for Sample Quality Assessment Using the Agilent Fragment Analyzer Systems

The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for a broad variety of applications, including next-generation sequencing (NGS) libraries, RNA samples, PCR amplicons, and CRISPR workflows.

Application Notes
English
03 Feb 2022
3.76 MB
PDF

Application Notes

Brochures

Case Studies

Citations

Data Sheets

Technical Overviews

Support

User Manuals

Agilent DNF-477 HS Small Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Small Fragment kit is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.

User Manuals
English
10 Apr 2025
357.31 KB
PDF

Agilent DNF-476 Small Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Small Fragment kit (p/n DNF-476-0500) is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next-Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.

User Manuals
English
10 Apr 2025
386.69 KB
PDF

Agilent DNF-474 HS NGS Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-474 HS NGS Fragment assay is designed for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.

User Manuals
English
10 Apr 2025
352.15 KB
PDF

Agilent DNF-473 NGS Fragment Kit (1-6000 bp) Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The NGS Fragment kit (1–6000 bp) (p/n DNF-473- 0500 and DNF-473-1000) is used for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.

User Manuals
English
10 Apr 2025
390.24 KB
PDF

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