Large Fragment Analysis Kits

Evaluating the quality of large DNA fragments and smears is essential for various nucleic acid applications, including long-read next-generation sequencing (NGS). The workflow for long-read NGS includes multiple quality assurance steps. The Agilent Fragment Analyzer systems, combined with the HS Large Fragment 50 kb kit, are highly recommended for their capability in both sizing and quantification for fragments up to 50 kb, substantially reducing the time and sample input needed for routine quality checks.

The Large Fragment kits are highly effective in separating both long-read sequencing libraries and DNA fragments or smears. The HS Extended Large Fragment DNA Ladder includes a 48.5 kb fragment that remarkably extends the sizing capability to include fragments up to 50 kb. When using the Fragment Analyzer systems in combination with the HS Large Fragment 50 kb kit, pulsed-field gel electrophoresis can be substituted at several key quality control checkpoints.