Bioanalyzer Small RNA Analysis
RUO
Small RNA electrophoresis with the 2100 Bioanalyzer System allows separation and quantitation measurements of challenging RNAs, such as microRNA and exosomes, sized below 200 nt and are often present in limited abundance. The Bioanalyzer Small RNA assay provides a fast and sensitive analysis to resolve small nucleic acid samples in the size range of 6 to 150 nt.
In addition, the Bioanalyzer Small RNA assay is ideally suited for quality control of guide RNAs. In CRISPR/Cas workflows the synthesis of guide RNA (gRNA) is a key component for successful genome editing. gRNA created by in vitro transcription and purified may be run on the 2100 Bioanalyzer system and inspected for sizing, yield, impurities, and secondary structures.
In addition, the Bioanalyzer Small RNA assay is ideally suited for quality control of guide RNAs. In CRISPR/Cas workflows the synthesis of guide RNA (gRNA) is a key component for successful genome editing. gRNA created by in vitro transcription and purified may be run on the 2100 Bioanalyzer system and inspected for sizing, yield, impurities, and secondary structures.
For Research Use Only. Not for use in diagnostic procedures.
- Bioanalyzer RNA Kits & Reagents
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This product is not available for purchase by the general public.
Product Details
Features
- High resolution analysis, with a sizing range from 6 to 150 nucleotide, allows for separation of miRNA smears from tRNA.
- Minimal sample consumption. Only use as little as 50 pg of purified miRNA or 10 ng of total RNA for analysis in 1 μL volume.
- A faster and more sensitive alternative to agarose and polyacrylamide gels analysis for detection of small RNA.
- Compatibility with multiple sample types enables analysis of total RNA, fractionated, or synthetic samples.
Specifications
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News
Still Using the Bioanalyzer? Let’s Talk About What’s Next!
The 2100 Bioanalyzer instrument (model G2939B) has entered the support-only phase and is no longer available for purchase. We offer advanced alternatives like the TapeStation, Fragment Analyzer, and ProteoAnalyzer systems.
- 14 May 2025
How It Works
Literature
- Key Literature
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Analysis of small RNAs with the Agilent 2100 bioanalyzer
NA
- Application Notes
- English
- 13 Jul 2006
- 164.07 KB
Comparison of Small RNA Analysis using the Agilent Bioanalyzer and Agilent Fragment Analyzer Systems
In this paper, samples are compared across both the Agilent 2100 Bioanalyzer and the Fragment Analyzer Small RNA kits to demonstrate the equivalency of the systems to analyze small RNAs.
- Application Notes
- English
- 04 May 2022
- 325.60 KB
- Application Notes
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