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Advance BioHPLC Performance

Advance your confidence for biotherapeutics

Agilent AdvanceBio columns are designed to enhance the accuracy and speed of your biomolecule characterization, including monoclonal antibodies (mAbs), other proteins, peptides and synthetic oligonucleotides.

Featured Product:
NEW Agilent AdvanceBio Amino Acid Analysis for Biomolecules

For Research Use Only. Not for use in diagnostic procedures.

End uncertainty

Eliminate slow separations, poor resolution, and other mAb characterization roadblocks with the new Agilent AdvanceBio SEC columns

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Resolve mAbs faster and better

Agilent AdvanceBio RP-mAb columns provide sharper, narrower peaks for confident separations of monoclonal antibodies

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Advance your glycan mapping

Achieve reproducible, high-resolution identification of 2-AB labeled N-linked glycans with the AdvanceBio Glycan Mapping columns, sample preparation and standards

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Advanced Amino Acid Analysis

Achieve fast, sensitive, reproducible separation of amino acids in biological samples with Agilent AdvanceBio Amino Acid Analysis (AAA) columns, reagents, and standards

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One column for discovery and QC

Reliably characterize primary sequence and detect post-translational modifications (PTMs) with new Agilent AdvanceBio Peptide Plus columns

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New AdvanceBio Columns Improve Laboratory Workflows

Biotech Embroiders Intricate Glycopatterns


AdvanceBio Webinar Series

Visit our biomolecule webinars hub for a full list of topics, dates, and times
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Recorded Webinars

CHROMacademy webinar: Method Development for Ion Exchange Using Dynamic Buffer Generation
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Tips and Tricks to Achieve Fast, Sensitive, and Reproducible Separation of Amino Acids
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CHROMacademy webinar: Choosing the Correct Column and Mobile Phase for LC/MS Characterization of Biologics
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CHROMacademy webinar: Making the Right Choices in SEC Method Development
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Reliable Amino Acid Analysis using the AdvanceBio AAA kit and automated derivatization
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Versatile PLRP-S Columns for a reversed phase solution for the characterization of monoclonal antibodies
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LabRoots webinar: Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates
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Development of analysis methods for monoclonal antibodies using innovative superficially porous particle biocolumns
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Agilent Biopharma Solutions

Moving the field of Biopharma forward

Protein Identification, Impurity Profiling, and Post Translational Modifications

Reversed-phase columns to boost your accuracy and productivity

Agilent offers the most comprehensive range of optimized wide-pore, reversed-phase BioHPLC columns, all backed by technical support experts and application chemists around the globe. The family includes 1.8, 3.5, and 5 µm totally porous particles for pressures from 400 to 1200 bar; 3.5 and 5 µm Poroshell (superficially porous) particles for UHPLC separations at lower pressure, and polymeric columns for analysis under the most extreme conditions.

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Charged Variants

Ion-exchange columns to characterize charged variants with speed and confidence

Whether your goal is to characterize the next biopharmaceutical or isolate a target protein, Agilent can help you overcome the challenges of isolating and identifying charged variants of proteins. A wide range of pore sizes means you can effectively separate proteins and charged variants of any size.

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Protein Aggregates and Degradants

Size exclusion columns for fast, high-resolution separation for affinity chromatography

Agilent BioHPLC columns for SEC are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions giving you the choices you need to perfect separations based on your analytes and method parameters.

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Glycans and Glycopeptides

A complete consumables workflow for the preparation and analysis of 2-AB labeled N-linked glycans, sample preparation, standards, and columns

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Special Offers

Glycan mapping resource kit

Includes application briefs, flyer, and a free poster.

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Amino Acid Analysis

Fast, sensitive, reproducible amino acid analysis

Combined with Agilent InfinityLab LC Series instruments, Agilent’s new AdvanceBio Amino Acid Analysis (AAA) provides a complete solution for amino acid analysis.

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AdvanceBio AAA ready-to-use amino acid reagents and standards kit including:

  • Agilent’s proven ortho-phthalaldehyde/
    chloroformate (OPA/FMOC) reagent for amino acid derivatization
  • AdvanceBio AAA ready-to-use amino acid standards kit

AdvanceBio AAA LC columns, based on Agilent’s innovative 2.7 µm Poroshell technology, for:

  • Improved reliability of results—high-resolution separations delivered by efficient Poroshell particle morphology.
  • Reduced costs—long column lifetimes from robust, high-pH resistant, chemically modified silica.
  • Increased flexibility—compatibility with both HPLC and UHPLC systems via 2.7 µm diameter particles.

Special Offers

Learn more with the Amino Acid Analysis Resource Kit, which includes:

  • Amino Acid Application Note
  • Proven Agilent Amino Acid How To Guide
  • Automated Amino Acid Analysis Technical Poster
  • Agilent AAA Workflow Flyer
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Titer Determination

Affinity columns for rapid determination of mAb concentrations in fermentation broths and purification of small quantities for characterization
  • Large dynamic range for quantitation over a wide range of product concentrations
  • Ideal for clone selection and optimization
  • Agilent Bio-Monolith Protein A with specificity for IgG subclasses 1 and 2
  • Agilent Bio-Monolith Protein G for IgG subclass 3


High-resolution oligonucleotide analysis with long column lifetime

A key challenge in the development and manufacture of oligonucleotide therapeutics is the need for analytical methods to separate and identify impurities. Agilent AdvanceBio Oligonucleotide columns and standards offer reliable high-resolution oligonucleotide analysis with long column lifetime.

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Desalting Cartridges

Agilent AdvanceBio Desalting-RP Cartridges

With new AdvanceBio Desalting-RP cartridges, you can quickly and efficiently remove salt ions online before MS detection. These flexible, cartridge-style columns can be used in two ways:

  • On their own with any LC system to desalt collected fractions
  • On an Agilent 1290 Infinity II 2D-LC system as a second-dimension desalt after a first-dimension affinity, ion exchange, or size exclusion separation

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For Research Use Only. Not for use in diagnostic procedures.