![Immunotherapy Immunotherapy](/cs/publishingimages/immunotherapy-solution.jpg)
Immunotherapy
The next generation of immune cell therapies requires a new generation of analysis tools
A safe, potent, and persistent immune cell product depends on developers mustering the full repertoire of immune cell function; activation, proliferation, cell fate, cytotoxic killing, immunomodulation, and memory. All of which must be accomplished in the ever-changing, suppressive, and toxic tumor microenvironment.
Agilent is dedicated to supporting these next-generation therapies, providing key technologies to enable high-efficiency gene editing and assessment of real-time cell function, phenotype, and fate. Used together, this tool bench provides the capability to measure and control immune cell function. Thus enabling translational researchers and developers to achieve the necessary level of therapeutic potency and safety.
Uncover Agilent’s next generation of solutions for immune and CAR T Cell therapy.
For Research Use Only. Not for use in diagnostic procedures.
Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
- PDF/ Found In: Application Notes
- Date : 22 Dec 2016
- File Size : 634.88 KB
Assessing functional metabolism using cells sourced from blood
- PDF/ Found In: Application Notes
- Date : 04 Jan 2017
- File Size : 643.12 KB
- Application Notes
-
A Novel Real-Time Co-Culture Assay Using xCelligence RTCA eSight for Immune Cell Invasion and Cytotoxicity
This application note describes a novel real-time co-culture assay using RTCA eSight for interrogating NK92 immune cell invasion and cytotoxicity and migratory kinetic profiles based on ECM and effects of TME.
- Application Notes
- English
- 01 Dec 2023
- 3.22 MB
Bispecific T Cell Engager (BiTE) Mediated Cancer Cell Lysis Assay
This application note evaluates the Agilent xCELLigence RTCA eSight as a tool to monitor BiTE antibody-mediated cancer cell lysis.
- Application Notes
- English
- 26 Apr 2023
- 2.44 MB
Real-Time Potency Assay for CART Cell Killing of Adherent Cancer Cells
Using the Agilent xCELLigence RTCA eSight to continuously monitor CAR T cell-mediatedkilling of cancer cells over the course of multiplae days.
- Application Notes
- English
- 11 Nov 2020
- 3.61 MB
Real-Time Specificity and Potency Assessment of Human Papilloma Virus Specific Engineered T Cells
Using the Agilent xCELLigence RTCA system to assess both the selectivity and potency of the TCR-engineered TILs toward HLA restricted cells.
- Application Notes
- English
- 21 Jul 2020
- 1.38 MB
Analysis of Phosphorylated STAT Protein Signaling in Lymphocytes Using Flow Cytometry
Examines the phosphorylation of signal transducer and activator of transcription (STAT) proteins in response to cytokine stimulation using a phosphoflow technique.
- Application Notes
- English
- 02 Jul 2020
- 1.13 MB
Assessing functional metabolism using cells sourced from blood
Real-time, kinetic measurements of functional metabolism in cells sourced from blood link mitochondrial function to disease diagnosis and progression.
- Application Notes
- English
- 04 Jan 2017
- 643.12 KB
Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
A real-time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.
- Application Notes
- English
- 22 Dec 2016
- 634.88 KB
Deciphering the Impact of Antigen Density on Tumor Targeting Therapeutics
In this application note, Antibody Analytics (Motherwell, UK) integrates IndEx-2, an advanced in vitro cell-based platform engineered to modulate the expression of one or two target antigens across a range of biologically relevant levels, with the Agilent xCELLigence Real Time Cell Analyzer (RTCA), Agilent NovoCyte flow cytometer, and cytokine release measurement protocols, to determine the antigen density thresholds required for the activation of potential immunotherapeutic candidates.
- Application Notes
- English
- 06 Jun 2024
- 2.21 MB
In-Depth Characterization of the Major Leukocyte Cell Subsets in Human Peripheral Mononuclear Cells
A 45-color spectral flow cytometry panel was designed for the Agilent NovoCyte Opteon spectral flow cytometer to analyze the distribution of immune cells.
- Application Notes
- English
- 30 May 2024
- 7.94 MB
- Brochures
- Case Studies
- Flyers
- Posters
- White Papers
适应性免疫细胞功能的代谢调节
在本次网络研讨会中,Elevatebio 的高级科学家 Sandra Milasta 博士将介绍代谢通路如何维持淋巴细胞存活、驱动记忆与效应功能,以及这些发现对新一代 T 细胞疗法开发的影响。人们对免疫疗法领域的关注日益提升,并希望了解我们是否可以通过靶向 T 细胞代谢来塑造 T 细胞应答。
模拟人 T 细胞的抗肿瘤功能
小鼠模型对于确立 T 细胞在抗肿瘤应答中的作用至关重要,但是由于许多人类抗体缺乏与小鼠受体的交叉反应,因此它们在转化研究中用于候选疗法测试的效用有限。然而,大多数用于研究抗肿瘤 T 细胞应答的基于人类细胞的模型都存在显著的生物学限制或实际限制,实用性有限。
在本次网络研讨会中,来自阿斯利康的 Michael Overstreet 博士将介绍基于抗病毒记忆 T 细胞的模型系统的构建,用于研究 HLA 相合人肿瘤细胞的抗原特异性 T 细胞介导的细胞毒性作用。本研讨会将展示如何使用实时仪器优化该模型,从而对影响肿瘤细胞和 T 细胞相互作用的动态变量之间的关系产生新的理解。