大片段分析试剂盒
在长读长测序文库的 QC 工作流程中有若干个质量保证步骤。重要的样品 QC 检查点包括：检查起始 DNA 材料的降解程度、分析剪切 DNA 片段确保正确分子量范围，以及测定最终长读长测序文库的分子量和含量。
- Fragment Analyzer Systems DNA Analysis Kits
Product Details
- 宽 DNA 片段浓度范围 — 使用大片段试剂盒分析浓度范围为 0.1–10 ng/µL 的 DNA 片段，使用 HS 大片段试剂盒分析浓度范围为 5–600 pg/µL 的 DNA 片段
- 宽 DNA 弥散条带分析范围 — 使用大片段试剂盒定量分析 5–100 ng/µL 的 DNA 弥散条带，HS 大片段试剂盒允许 50–5000 pg/µL 的较低浓度范围
- 低样品量 — 仅需 2 µL 样品，可大幅减少 QC 步骤的样品损失
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- Key Literature
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Quality Control in the PacBio HiFi Whole Genome Sequencing Library
Accurate and reliable next-generation sequencing (NGS) results rely on starting with high-quality samples.
- Application Notes
- English
- 02 Jul 2025
- 1.77 MB
Target Capture Long-Read Sequencing Using the Agilent SureSelect XT HS2 Target Enrichment System
This application note introduces a method for target capture (long-read) sequencing using the Agilent SureSelect XT HS2 target enrichment system with some protocol modifications.
- Application Notes
- English
- 23 Aug 2024
- 1.63 MB
- Application Notes
- Brochures
- Case Studies
- Citations
- Data Sheets
- User Manuals
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Agilent DNF-464 HS Large Fragment Kit Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Large Fragment 50 kb kit (500 Samples) (Part # DNF-464-0500) is designed for the sizing and quantification of medium to high molecular weight dsDNA smears/fragments.
- User Manuals
- English
- 30 Apr 2025
- 361.58 KB
Agilent DNF-492 Large Fragment Kit Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Large Fragment kit (p/n DNF-492-0500 and DNF-492-1000) is designed for the sizing and quantification of small to mid-range molecular weight dsDNA smears/fragments. Example applications include quality control of Next Generation Sequencing (NGS) libraries.
- User Manuals
- English
- 10 Apr 2025
- 377.85 KB
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