基因组 DNA 165 kb 试剂盒
长读长测序文库制备涉及到多个 gDNA 和文库质量控制步骤。与标准的 16 小时脉冲场凝胶电泳 (PFGE) 方案相比，基因组 DNA 165 kb 试剂盒仅需 1.5 小时即可提供结果，大大减少了长读长测序文库工作流程所需的时间和工作量。
- Femto Pulse System DNA Analysis Kits
Product Details
- 可靠的分子量测定 — 在 1300 bp-165 kb 范围内，确保对 HMW gDNA 样品实现高准确度与高精度的分子量测定
- 快速获得结果 — 经优化的脉冲场分离方法可缩短分析时间，无需进行过夜 PFGE 分离
- 质量指标 — 使用基因组质量值 (GQN) 快速评估 HMW gDNA 的完整性。
- 超高灵敏度检测 — 可使用低至 5 pg/µL 的起始浓度
- 低样品量 — 仅需 2 µL 样品，节省样品用于下游分析
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| 片段大小测定准确度 |
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- Key Literature
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Quality Control in the PacBio HiFi Whole Genome Sequencing Library
Accurate and reliable next-generation sequencing (NGS) results rely on starting with high-quality samples.
- Application Notes
- English
- 02 Jul 2025
- 1.77 MB
Visualizing DNA for Long-Read Sequencing by Moles, Not Mass
Visualization of the sample by moles using the Femto Pulse system can provide a clearer assessment of the length distribution, and better predict sequencing results.
- Application Notes
- English
- 07 Apr 2024
- 645.41 KB
Comparison of Constant- and Pulsed-Field Electrophoresis Technologies for Analysis of High Molecular Weight and Large DNA Fragments
This application note demonstrates the use of the Agilent Fragment Analyzer and Femto Pulse systems for analysis of high-molecular-weight and large DNA fragments.
- Application Notes
- English
- 22 Jun 2023
- 540.88 KB
- Application Notes
- Brochures
- Case Studies
- Data Sheets
- User Manuals
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Agilent Genomic DNA 165 kb Kit Quick Guide for the Femto Pulse System
This Quick Guide lists the necessary steps to analyze HMW, low concentration DNA smears and/or fragments from 1.3-165 kb with the Agilent Genomic DNA 165 kb kit and the Agilent Femto Pulse system.
- User Manuals
- English
- 02 Jun 2025
- 386.94 KB
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