Skip to main content

Selection Antibiotics - Details & Specifications

G418 Sulfate
  • Selects eukaryotic cells stably transfected with neomycin-resistant genes
  • Useful as a selective agent in gene transfer experiments
  • Supplied as a crystalline solid or aqueous solution

Supplied as a crystalline solid or aqueous solution, C20 H40 ON4 ยท 2H2 SO4

MOLECULAR WEIGHT: 496.5 (base) and 692.7 (sulfate)

CONCENTRATION: For the selection of Neor cells use 10 to 500 mg/L. Optimal concentrations are determined experimentally and vary by cell type.

TESTING CONDITIONS: G418 Sulfate is quality tested by applying the resuspended sulfate to NIH3T3 cells that have been transfected with a plasmid, pWLneo, containing the neomycin-resistant gene. After 2 weeks of treatment, the transfection is screened for the formation of stable colonies.

 

Hygromycin B
  • Selects eukaryotic cells stably transfected with hygromycin b-resistant genes
  • Useful as a selective agent in gene transfer experiments
  • Supplied in a liquid format

Supplied as a carmel-colored liquid, C20 H37 N3 O13

Molecular Weight: 527


For Research Use Only. Not for use in diagnostic procedures