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RNA Isolation Kits - Details & Specifications

Total RNA Isolation Mini Kit
  • Sample required per isolation is 2.5 - 30 mg tissue or 5 x 105 - 1 x 106 cells
  • Delivers up to a 1,000-fold reduction of genomic DNA contamination without DNase treatment
  • Handles a wide range of sample types (e.g., mammalian cells and tissues including fibrous samples, yeast, and bacteria).
  • An optional, on-column DNase digestion step is available that easily integrates into the workflow

The Agilent Total RNA Isolation Mini Kit uses a straightforward, spin-column method to deliver total RNA for use in gene expression and other downstream analyses. A unique prefiltration column simultaneously clarifies and homogenizes viscous samples, facilitating simultaneous RNA isolation and genomic DNA removal. The kit delivers significant reduction of genomic DNA contamination compared to silica-based kits and it's easier to use and simpler than conventional DNAse-based methods.



Kit Contents

Columns Mini-filtration columns & 2 mL collection tube (color-coded natural), 10 packs, 5 columns/pac
Mini-filtration columns & 2 mL collection tube (color-coded blue), 10 packs, 5 columns/pack
RNase-free collection tubes, 10 packs, 5 tubes/pack
Reagents
(3 Bottles)
Lysis Solution, 50 mL
Wash Solution, 12 mL
Nuclease-free water, 25 mL



Plant RNA Isolation Mini Kit
  • Sample required per isolation is 25 – 100 mg tissue
  • Isolate higher purity RNA even from notoriously challenging sources such as waxy and oil tissues (e.g., seeds & conifer needles) or polysaccharide-containing storage tissues (e.g., tubers)
  • Up to a 10,000-fold reduction in genomic DNA contamination compared to kits that don't include DNase
  • Resulting RNA is free of known inhibitors of downstream reactions, such as carbohydrates, waxes, and secondary metabolites

The Agilent Plant RNA Isolation Mini Kit delivers intact and high-purity total RNA for use in gene expression and other downstream analyses. This kit is a straightforward, spin-column format that is both DNAse- and phenol-free. It has been specially formulated for plant samples from a wide variety of species and tissue types.



Kit Contents

Columns Mini-filtration columns & 2 mL collection tube (color-coded natural), 10 packs, 5 columns/pack
Mini-filtration columns & 2 mL collection tube (color-coded blue), 10 packs, 5 columns/pack
RNase-free collection tubes, 10 packs, 5 tubes/pack
Reagents
(3 Bottles)
Extraction solution, 50 mL
Wash solution, 19.5 mL
Nuclease-free water, 25 mL


For Research Use Only. Not for use in diagnostic procedures