RNA Isolation Kits - Details & Specifications

• Sample required per isolation is 2.5 - 30 mg tissue or 5 x 10⁵ - 1 x 10⁶ cells
• Delivers up to a 1,000-fold reduction of genomic DNA contamination without DNase treatment
• Handles a wide range of sample types (e.g., mammalian cells and tissues including fibrous samples, yeast, and bacteria).
• An optional, on-column DNase digestion step is available that easily integrates into the workflow

The Agilent Total RNA Isolation Mini Kit uses a straightforward, spin-column method to deliver total RNA for use in gene expression and other downstream analyses. A unique prefiltration column simultaneously clarifies and homogenizes viscous samples, facilitating simultaneous RNA isolation and genomic DNA removal. The kit delivers significant reduction of genomic DNA contamination compared to silica-based kits and it's easier to use and simpler than conventional DNAse-based methods.

Kit Contents

• Sample required per isolation is 25 – 100 mg tissue
• Isolate higher purity RNA even from notoriously challenging sources such as waxy and oil tissues (e.g., seeds & conifer needles) or polysaccharide-containing storage tissues (e.g., tubers)
• Up to a 10,000-fold reduction in genomic DNA contamination compared to kits that don't include DNase
• Resulting RNA is free of known inhibitors of downstream reactions, such as carbohydrates, waxes, and secondary metabolites

The Agilent Plant RNA Isolation Mini Kit delivers intact and high-purity total RNA for use in gene expression and other downstream analyses. This kit is a straightforward, spin-column format that is both DNAse- and phenol-free. It has been specially formulated for plant samples from a wide variety of species and tissue types.

Kit Contents

For Research Use Only. Not for use in diagnostic procedures