Sample required per isolation is 2.5 - 30 mg tissue or 5 x 105 - 1 x 106 cells
Delivers up to a 1,000-fold reduction of genomic DNA contamination without DNase treatment
Handles a wide range of sample types (e.g., mammalian cells and tissues including fibrous samples, yeast, and bacteria).
An optional, on-column DNase digestion step is available that easily integrates into the workflow
The Agilent Total RNA Isolation Mini Kit uses a straightforward, spin-column method to deliver total RNA for use in gene expression and other downstream analyses. A unique prefiltration column simultaneously clarifies and homogenizes viscous samples, facilitating simultaneous RNA isolation and genomic DNA removal. The kit delivers significant reduction of genomic DNA contamination compared to silica-based kits and it's easier to use and simpler than conventional DNAse-based methods.
Sample required per isolation is 25 – 100 mg tissue
Isolate higher purity RNA even from notoriously challenging sources such as waxy and oil tissues (e.g., seeds & conifer needles) or polysaccharide-containing storage tissues (e.g., tubers)
Up to a 10,000-fold reduction in genomic DNA contamination compared to kits that don't include DNase
Resulting RNA is free of known inhibitors of downstream reactions, such as carbohydrates, waxes, and secondary metabolites
The Agilent Plant RNA Isolation Mini Kit delivers intact and high-purity total RNA for use in gene expression and other downstream analyses. This kit is a straightforward, spin-column format that is both DNAse- and phenol-free. It has been specially formulated for plant samples from a wide variety of species and tissue types.