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Restriction Enzymes - Details & Specifications

2015-08-18Stringent Quality Control
Quality control is achieved utilizing pBluescript phagemid cloning assay, which tests the overall quality of restriction enzymes used when cloning into the multiple cloning site of the pBluescript II phagemid vector. pBluescript II DNA is linearized with the specific restriction enzyme and then ligated. The ligated phagemid is transformed into Epicurian Coli XL1-Blue supercompetent cells and plated on LB/ampicillin plates with X-gal and IPTG for color selection. Stringent specifications are set for the number of false-positive colonies that can be generated following transformation.

Buffers
With each restriction enzyme purchase, we include an optimal (10X) buffer and/or a 10X concentrate of Universal buffer. Universal buffer is optimal for a large percentage of the restriction enzymes that we offer. It is used in one of four dilutions (0.5X, 1.0X, 1.5X or 2.0X) and is an excellent buffer for use in double-digest reactions. 10X buffers must be carefully formulated with regard to pH. Our buffers are subjected to strict quality control for maximum utility across our entire line of restriction enzymes.

HC
HC indicates high-concentration enzymes.


For Research Use Only. Not for use in diagnostic procedures