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FAME Columns

Speed Up Your FAME
Analysis with Confidence

Agilent GC columns for FAMEs,
fatty acids, and triglycerides

oil-based dressing

Perform fast analysis of FAMEs with superior resolution—even for challenging cis-trans isomers

Agilent J&W DB-FastFAME columns have been cyanopropyl-phase (G48) engineered so that you can quickly and selectively separate saturated and polyunsaturated FAMEs—including key cis-trans isomers. What’s more, you don’t have to sacrifice the resolution achieved with traditional 100-meter-long columns.

With these innovative columns, you can separate a traditional FAME mixture in under eight minutes. This includes C18:1 and C18:2 cis-trans isomers and FAMEs found in milk fat, vegetable oil, and fish oil—such as DPA and EPA.

Agilent J&W DB-FATWAX Ultra Inert GC columns use proprietary Ultra Inert technology. What’s more, DB-FATWAX is the only WAX-type phase that can produce symmetric peaks for even challenging polar compounds, such as free fatty acids. This feature improves inertness, thermal stability, and column lifetime compared to traditional WAX columns.

These innovative columns are designed to separate fatty acid methyl esters (FAMEs), fatty acid ethyl esters (FAEEs), and fatty acids. You can also use them with confidence to analyze Omega 3 and 6 and free fatty acids (C4-C16).

You also gain these advantages:

  • Columns are individually tested with a FAME mixture to ensure reproducible FAME equivalent chain length (ECL) values.
  • They comply with AOAC, AOCS, and IOC regulatory methods.
  • The cyanopropyl phase of DB-FastFAME is bonded, cross-linked, and solvent rinseable.
  • Intuvo configurations are available.

Eight-minute separation of a FAME mix with DB-FastFAME columns

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Fast analysis of FAMEs in rapeseed oil using an Intuvo DB-FastFAME column

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Productivity tool kit

productivity tool kit materials


  • On-demand webinar: Learn how to select the most efficient GC column—and how to enable baseline separation of critical FAMEs and fatty acids on a single column
  • Brochure: Comprehensive Analysis of FAMEs, Fatty Acids, and Triglycerides
  • Application notes: FAMEs and fatty acids analysis
Download now

Eight-minute separation of a FAME mix with DB-FastFAME columns


Fast analysis of FAMEs in rapeseed oil using an Intuvo DB-FastFAME column


Select the right column for your samples

Type of Fatty Acid CP-FFAP CB DB-FATWAX UI DB-FastFAME CP-Sil 88 for FAME/HP-88 Select FAME CP-TAP CB for Triglycerides ChromSpher Lipids (LC)
Short-chain free fatty acids (C2-C6) Yes Yes No No No No No
Medium-chain free fatty acids (C6-C16) Yes Yes No No No No No
Long-chain free fatty acids (C16-C24) Yes No No No No No No
Omega 3 & 6 FAMEs No Yes Yes Yes Yes No No
FAMEs by degree of saturation No Yes No No No No No
FAME groups of cis and trans isomers No No Yes Yes Yes No No
FAMEs geometrical positional isomers No No No Yes Yes No No
Cholesterol and triglycerides No No No No No Yes Yes
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To advance your analysis of FAMEs and fatty acids, complete this form.