Quickly determine the quality of your FFPE RNA samples with the Automated Electrophoresis Portfolio
The Automated Electrophoresis systems from Agilent utilize a quality metric, DV200, to address the unique QC challenges of FFPE RNA. The DV200 provides researchers with a tool to reliably classify degraded RNA by size and effectively parse samples suitable for NGS from unsuitable samples, conserving time and costs. Depending on the level of degradation and the DV200 score, researchers can modify their workflows to still enable the use of FFPE samples in downstream applications. For example, when a specific size is needed and the sample is slightly degraded, the shearing protocol can be adjusted to reach that size. Take advantage of automated evaluation of RNA samples using the DV200 quality metric with the Bioanalyzer, TapeStation, and Fragment Analyzer systems.
Store the method/configuration files for the RNA kit (15 nt) or the HS RNA kit (15 nt) in the Configuration folder of the ProSize data analysis software under: C:\ProSize data analysis software\Configurations\33cm
To apply the DV200 calculation to an existing data file:
Open Fragment Analyzer run in ProSize data analysis software
Click “Option” Menu → Edit Configurations
Click Smear Analysis → Enter start size (e.g. 200 nt) and end size (e.g. 10000 nt)
Click Load → a second Advance Setting window will open
Click “Load” → Open the Configurations file folder “33cm” (C:\ProSize data analysis software\ Configurations\33cm)
Select the desired Configuration file – Okay – Apply
Click back on “Set individual parameters” Icon → Smear analysis tab → % total column
When DV200 regions are defined, the value is provided under the Smear analysis tab in the % of total column for each sample.
For Research Use Only. Not for use in diagnostic procedures.