Skip to main content

DV200 determination for FFPE RNA samples

Quickly determine the quality of your FFPE RNA samples | Agilent 2100 Bioanalyzer System - DV200 Determination
Quickly determine the quality of your FFPE RNA samples

On the 2100 Bioanalyzer or 4200 TapeStation system, quickly calculate the DV200 value (the percentage of fragments >200 nucleotides) of your sample. Many RNA expression protocols, such as Agilent's SureSelectXT RNA Direct, will provide guidance for FFPE sample processing dependent on this value.

Bioanalyzer
Tapestation
Want to know more?

Agilent 2100 Bioanalyzer System

Simplified DV200 Evaluation with the Agilent 2100 Bioanalyzer System
Read Technical Overview

Agilent 4200 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays
Read Technical Overview
Instructions for use
Agilent 2100 Bioanalyzer system

The DV200 assays can be used with the 2100 Expert Software B.02.08 or higher.

  • Download the latest version of the 2100 Expert Software
  • Store the DV200 RNA Nano.xsy or DV200 RNA Pico.sxy file in the 2100 Expert Software RNA assay folder:
    c:\Program Files (x86)\
    Agilent\2100bioanalyzer\
    2100expert\assays\RNA

To apply the DV200calculation to an existing data file:

  1. Open the data file with the 2100 Expert Software
  2. Navigate to the Assay Properties tab
  3. Click Import Setpoints and select the appropriate .xsy file (DV200 RNA Nano or DV200 RNA Pico, dependent on the data file opened)

Assay parameters will be applied to the data file. Refer to the Technical Overview for more details.

Agilent 4200 TapeStation system

The DV200 assays can be used with the TapeStation Analysis software A02.02 or higher.

  1. Open a data file with the TapeStation Analysis Software
  2. Click Regions
  3. Click Regions Settings
  4. Enter the lower (e.g. 200 nt) and upper limits (e.g. 10000 nt) of the region in the dialog box. Type DV200as region comment.
  5. To apply the DV200region to all samples in the active file, select This File. To apply the DV200region to all files of the same assay as the active file, select Assay Files.

When DV200 regions are defined, the value is provided in the region table in the % of total column for each sample.

Refer to the Technical Overview more details.

For Research Use Only. Not for Use in diagnostic procedures.
PR7000-1548