Pfu DNA Ligase
RUO
Pfu DNA ligase is a recombinant enzyme derived from pyrococcus furiosus hyperthermophilic marine archaebacterium. The recombinant DNA Ligase has a higher ligation specificity and lower background than T4 DNA ligase.
Pfu DNA ligase catalyzes linkages of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45°C to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. The ligase is highly thermostable and it increases the reliability of LCR by permitting higher melting temperatures.
Pfu DNA ligase catalyzes linkages of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45°C to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. The ligase is highly thermostable and it increases the reliability of LCR by permitting higher melting temperatures.
For Research Use Only. Not for use in diagnostic procedures.
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Product Details
Features
- Featuring higher ligation specificity and lower background than Tth DNA ligase
- Eliminates LCR template-denaturation step
- The ligase is highly thermostable and features a half-life of over 60 minutes at 95°C
- Increases reliability of LCR by permitting higher melt temperatures
- Eliminates the LCR template-predenaturation step meaning that less enzyme is required for LCR
- Operates at an optimum temperature near 70°C for nick-sealing reactions
