The DNA Ladder contains fragments from 2 to 10 kb at 1 kb increments and an additional higher band at 12 kb as well as bands at 250, 500, 750 and 1500 bp.
The higher bands are brighter than those at the lower end, with the transition to higher intensity at the 2 kb band serving as a useful reference point. Each fragment contains a 5' overhang (TTAA) that can be radioactively labeled using the Klenow Fill-In Kit. DNA fragments may also be detected via nonradioactive Southern blot analysis by incorporating fluor-modified nucleotides into the DNA prior to electrophoresis and using a tan Illuminator Chemiluminescent Detection System.
For Research Use Only. Not for use in diagnostic procedures.