SP6 RNA Polymerase
RUO
SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and incorporates 35S, 32P and 33P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone.
SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity.
Unit definition: One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C.
Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25-µl volume. Incubate 60 minutes at 37°C.
SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity.
Unit definition: One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C.
Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25-µl volume. Incubate 60 minutes at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
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Product Details
Features
- Probes for nucleic acid hybridization
- Templates for in vitro translation
- Substrates for RNA processing studies
- Exon and intron mapping of genomic DNA
