RNase Block
RUO
RNase Block Ribonuclease Inhibitor is a 50-kDa protein that specifically inhibits common eukaryotic RNases including RNase A, RNase B, and RNase C. It is a recombinant ribonuclease inhibitor derived by a molecular cloning technology that inactivates RNases by binding noncovalently to the RNase molecule.
For Research Use Only. Not for use in diagnostic procedures.
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Product Details
Features
- Only 25 to 40 U is required for a standard transcription reaction
- Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts with 40 U of RNase Block Ribonuclease Inhibitor for 1 hour at 37°C and by checking for the absence of degradation.
- Applications: RNA transcription, In vitro RNA translation, RNA capping, and Any application where eukaryotic RNase contamination is a potential problem
- RNase Block does not inhibit RNase H, RNase T1, T7, T3, or SP6 RNA polymerases, Taq DNA polymerase, AMV or MMLV reverse transcriptases
