Klenow, Full Size and Exonuclease Deficient
RUO
Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to complete fill-in of a 5 overhang to generate blunt ends, and allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha-32P]dNTP.
The Klenow Fill-in Kit contains Klenow fragment (125 U), dNTPs (separate 10 mM stocks), Klenow reaction buffer, predigested DNA, which can be used as a control, and sufficient material for 25 reactions.
The Klenow Fill-in Kit contains Klenow fragment (125 U), dNTPs (separate 10 mM stocks), Klenow reaction buffer, predigested DNA, which can be used as a control, and sufficient material for 25 reactions.
For Research Use Only. Not for use in diagnostic procedures.
Didn't find the product you need? Our lab is prepared to produce what you need. Request Custom Product Quote
Product Details
Features
- Perform a complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation
- Radioactively end label a 5 overhang with [alpha-32P]dNTP
- Includes predigested DNA that can be used as a control
- The sample DNA should be extracted with phenol–chloroform and ethanol-precipitated before performing a fill-in reaction.
- Add the Klenow polymerase to the reaction mixture last—after the DNA, fill-in buffer and dNTPs—and do not let the reaction mixture sit on ice for an extended period of time.
