Proteinase K

RUO

Widely used in DNA purification, Proteinase K is a broad-spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA. It cleaves peptide bonds where carbonyl groups are surrounded by aromatic, hydrophobic, or aliphatic amino acids.

Chromosomal DNA produced using Proteinase K is ideally suited for use in the following applications: pulsed field electrophoresis, chromosomal DNA for protein fingerprinting, and nuclease removal from DNA and RNA preparations.

For Research Use Only. Not for use in diagnostic procedures.

Modifying Enzymes

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Features

For general digestion of protein in biological samples
Lyophilized powder with specific activity of >30units/mg at 37ºC
Guaranteed stability for 6 months when stored correctly

Specifications


Concentration	30 U/µL
Incubation Time	Incubate 30 minutes at 37 °C
Physical Form	Lyophilized Powder
Reaction Conditions	50 mM Tris-HCl (pH 7.5), 5 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C
Storage Buffer	Store lyophilized or in 10 mM CaCl2 at 20°C
Unit Definition	One unit will hydrolyze casein to produce the absorbance equivalent to 1 µM of tyrosine/minute at pH 7.5, 37°C

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Proteinase K

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