XL1-Blue MR & MRF Competent Cells
RUO
XL-1 Blue MRF’ and XL-1 Blue MR competent cells allow high efficiency and representational cloning of methylated DNA. All known E. coli K12 restriction systems have been deleted. Use XL1-Blue MRF’ cells when cloning methylated cDNA or genomic DNA, or when cloning methylated PCR products.
When the F’ episome and blue-white screening are unnecessary, use XL1-Blue MR supercompetent cells. When cloning tetracycline-resistant plasmids, use XL1-Blue MRF’ Kan supercompetent cells to select for the F’ episome and provide a more intense blue color for blue-white screening.
When the F’ episome and blue-white screening are unnecessary, use XL1-Blue MR supercompetent cells. When cloning tetracycline-resistant plasmids, use XL1-Blue MRF’ Kan supercompetent cells to select for the F’ episome and provide a more intense blue color for blue-white screening.
For Research Use Only. Not for use in diagnostic procedures.
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Product Details
Features
- Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of ≥1 x 109 transformants/μg
- Transformation efficiency for XL1-Blue MRF Kan supercompetent cells of ≥1 x 109 transformants/μg
- Transformation efficiency for XL1-Blue MRF’ electroporation competent cells of ≥1 x 1010 transformants/µg
How It Works
