Difficult Cloning Competent Cell Pack
RUO
Difficult Cloning Competent Cell Packs contain three strains packaged together to increase lab efficiency, optimize protocols economically, and test multiple strains simultaneously. Use XL10-Gold Kanr ultracompetent cells to transform large plasmids or work with limited amounts of DNA.
Use SURE supercompetent cells to clone eukaryotic segments of DNA containing inverted repeats or secondary structures, such as Z-DNA. And use Able K competent cells to clone toxic genes present in any ColE1-derived plasmid and isolate sufficient DNA for most downstream applications.
Use SURE supercompetent cells to clone eukaryotic segments of DNA containing inverted repeats or secondary structures, such as Z-DNA. And use Able K competent cells to clone toxic genes present in any ColE1-derived plasmid and isolate sufficient DNA for most downstream applications.
For Research Use Only. Not for use in diagnostic procedures.
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Product Details
Features
- XL10-Gold ultracompetent cells, with the novel Hte (high transformation efficiency) phenotype, provide 25-fold higher transformation of ligated DNA compared to DH10B and XL2-Blue cells
- SURE supercompetent cells clone DNA segments unclonable' in conventional E. coli strains, with transformation efficiency of 1 x 10^9 transformations/µg DNA
- Able K competent cells reduce copy number of ColE1-derived plasmids 10-fold, decreasing the level of cloned gene products and enhancing probability of toxic clone propagation
