IQFISH Fast Hybridization Buffer is a formamide-free hybridization buffer that enables ISH hybridization to be completed in 1-1.5 hours. A complete in situ hybridization (ISH) assay on a formalin-fixed, paraffin-embedded (FFPE) sample can be completed in 4 hours enabling a half day sample-to-result workflow. This workflow offers many operation advantages such as increased turnaround time, same day repeat testing without impacting the quality of hybridization.
Standard formamide-based FISH hybridization buffer lowers the melting temperature of nucleic acids by targeting hydrogen bonds and interfering with DNA base pairing resulting in the denaturation of the DNA double strand. The interference slows down the following base pairing between the FISH probe and specimen DNA. However, IQFISH buffer destabilizes the DNA helix by diminishing hydrophobic base stacking (1). Minimized based paring interference enables fast binding between the ISH probe and specimen DNA.
IQFISH buffer is provided in three different size formats to meet the needs of varying testing volumes.