PNA ISH Detection Kit for fluorescein-labeled peptide nucleic acid (PNA) probes contains reagents necessary for performing an in situ hybridization, except for the specific probe. The kit contains proteinase K, negative and positive control probes, stringent wash solution, alkaline phosphatase-conjugated F(ab') fragment of rabbit anti-FITC, chromogenic substrate combined with an inhibitor of endogenous alkaline phosphatase (BCIP/NBT/levamisole), and Tris-buffered saline.