BCL2 IQFISH Break-Apart Probe is intended for the detection of rearrangements involving the BCL2 gene by fluorescence in situ hybridization (FISH). The probe is to be used on formalin-fixed, paraffin-embedded tissue sections.
Break apart probes consist of two child probes (3 and 5 probes), designed to hybridize on opposite sides of the translocation break point for a given gene, each labeled in a different color (Cy3 and FITC). These probes generate signals in normal cells that are closely matched in size and co-localized (2 fusion signals). Following a translocation, the signals are broken apart and no longer co-localize (for example: 1 orange-red, 1 green, 1 fusion).
BCL2 IQFISH Break-Apart Probe and corresponding protocol were developed using formalin-fixed, paraffin-embedded, lymph node tissue sections.
Child Probe 5' RD, Chr18; Start 61043686, Stop 61641631
Child Probe 3' GR, Chr18; Start 60140026, Stop 60763791