The ROS1 IQFISH Break-Apart Probe is intended for the detection of rearrangements involving the ROS1 gene by fluorescence in situ hybridization (FISH). The probe is to be used on lung paraffin-embedded tissue sections.
Break-apart probes consist of two child probes, designed to be on opposite sides of the translocation break point for a given gene, each labeled in a different color. These probes generate signals in normal cells that are closely matched in size and co-localized (2 fusion). Following a translocation, the signals are 'broken apart' and no longer co-localize (for example: 1 red, 1 green, 1 fusion).