Economical Multimode Microplate Reader, BioTek Synergy HTX

Features

Flexibility at an affordable price in a high-performance instrument. The hardware design and Gen6 software drive functionality and address a range of multimode applications without costly and often unused options
The combination of independent optics allows multimode measurements in a single instrument. Great sensitivity with filter-based fluorescence optics and monochromator-based UV-Vis absorbance optics for measurements without filters
Environmental controls and robust shaking profiles enable consistent data in live cell assay workflows, including temperature control to 50 °C
Excellent Alpha assay performance with sensitive fluorescence. Enhanced performance for many applications including important AlphaScreen and AlphaLISA assays
Fast inject/read assays use the available dual reagent injector module with angled tips. This operation delivers results for enzyme kinetics, Dual-Luciferase Reporter, and many assays. Simplify your workflow and save time in your critical assays
Microvolume nucleic acid and protein quantification with the Take3 microvolume plate

Specifications


Automation Compatibility	BioStack 3rd-party
Environment Control	4-Zone Incubation to 50 °C Condensation Control
Filter Detection Modes	Fluorescence Intensity Alpha Luminescence
Imager/Reader Options	Reagent injectors
Instrument Control	Computer Software
Monochromator Detection Modes	UV-Vis Absorbance Time Resolved Fluorescence (secondary)
Shaking	Linear Orbital
Supported Labware	6 to 384-well plates Take3/Take3 Trio PCR plates Cell culture dishes

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How It Works

Applications

Technology

Alpha detection

AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios.

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Absorbance

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.

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Fluorescence polarization

Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.

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