For use with both rabbit and mouse primary antibodies. The kit contains, in user-friendly dropper bottles, ready-to-use EnVision reagent. Also included is two-component high-sensitivity diaminobenzidine (DAB+) chromogenic substrate system.
The EnVision reagent of this kit is a peroxidase-conjugated polymer backbone, which, in addition, also carries secondary antibody molecules directed against rabbit and mouse immunoglobulins. The combination of several peroxidase molecules and several secondary antibody molecules on the same polymer provides a simple, yet sensitive, visualization system. Endogenous biotin will not affect staining results.
Other reagent provided with the kit:
Extra DAB for double application of chromogen.
Dako Autostainer template exists for this kit.