Intended for use in immunohistochemistry, immunoblotting, and ELISA. The cross-reaction with human immunoglobulins and fetal calf serum is negligible.
The antibody used for horseradish peroxidase conjugation reacts with rabbit immunoglobulins of all classes.
The immunoglobulin fraction has been solid-phase absorbed with human plasma protein and the absorbed specific antibody has been isolated by affinity chromatography on a column with rabbit immunoglobulins.The affinity-isolated antibody has then been conjugated with horseradish peroxidase (HRP) of very high specific enzymatic activity. Cross-reactions as determined by ELISA for the unconjugated antibody: Human immunoglobulins, less than 1%. Crossreactions with other immunoglobulins are typically: Cow, guinea pig, goat, mouse and rat, less than 0.5%. The crossreaction with fetal calf serum is typically below 0.1%.