Intended for use in immunohistochemistry, immunoblotting and ELISA. The cross-reaction with human, mouse and rat immunoglobulins, and fetal calf serum proteins is negligible. Enzyme-conjugated secondary antibodies (antibodies to animal immunoglobulins) are utilized either for the direct detection of animal immunoglobulins or for amplification of the reaction between a primary antibody and the target antigen. The cross-reaction with human, mouse and rat immunoglobulins, and fetal calf serum proteins is negligible.
The goat antibody used for horseradish peroxidase-conjugation reacts with rabbit immunoglobulins of all classes.
The immunoglobulin fraction has been solid-phase absorbed with human and cow serum proteins and the absorbed, specific antibody has been isolated by affinity chromatography on a column with rabbit immunoglobulins.
The cross-reaction with human serum is typically below the detection limit.