Intended for use in immunohisochemistry, immunoblotting, and ELISA. The cross-reaction with human immunoglobulins and fetal calf serum is negligible.
The HRP-conjugated antibody reacts with all mouse IgG subclasses and mouse IgM, and it will probably also react with other mouse immunoglobulins via their light chains.
Cross-reaction with human immunoglobulins and fetal calf serum has been removed by solid-phase absorption and absorbed specific antibody has been isolated by affinity chromatography on a column with mouse immunoglobulins. However, it should be noted that the cross-reaction with rabbit immunoglobulins is very low. The goat antibody used for horseradish peroxidase-conjugation may cross-react with immunoglobulins from other species.