R-Phycoerythrin (RPE) conjugates are used in flow cytometry, immunoassays, MHC tetramer assays and bead-based assays. RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy. Conventional conjugation techniques will not alter the spectral characteristics of this product. Using Agilent's highly purified RPE, we manufacture a variety of RPE conjugates for different applications. Among these are several different conjugates of RPE and streptavidin, which have different properties that make them especially suitable for one application or another (for instance, the optimal conjugates for bead-based assays are not usually the same as the optimal conjugates for MHC tetramer assays). We also offer conjugation kits and SMCC-activated RPE for simplified conjugation to antibodies and other thiol-containing proteins of interest.
This product is not available for purchase by the general public.
Product Details
Features
We have a range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates that differ in overall size. We recommend that you test our conjugates to find out which is the best for your system. Please contact Agilent to set up a sample to test in your application.
We have conjugates intended for use with Luminex and other bead based assays, Tetramer, and Flow cytometry applications.
PJRS301 is our newest conjugate, developed for long-term signal consistency.
PJRS25 is used in the NIH (Emory) Tetramer Core Facility protocol published online, along with our streptavidin-APC (allophycocyanin) conjugate PJ27S.
Also available are ANTI-FLAG® R-Phycoerythrin Conjugate [PJ315], Anti-GST (Type 1)-R-Phycoerythrin Conjugate [PJ312P1], and Goat Anti-Human IgG (Mouse Adsorbed)-R-Phycoerythrin Conjugate [PJR33].
We also offer a conjugation kit containing SMCC-activated RPE for simplified conjugation to antibodies and other sulfhydryl-containing proteins of interest. Custom conjugations to RPE are also available, please contact Agilent for further information.