APC Conjugates

Allophycocyanin (APC) conjugates (formerly ProZyme) are used in FRET screening, flow cytometry, immunoassays and MHC tetramer assays. APC is a highly absorptive fluorescent molecule that has excellent detectability. APC is usually used in an internally crosslinked form (Crosslinked APC) which is stable at the low concentrations required for most uses. Conventional conjugation techniques will not alter the spectral characteristics of this product. Using our highly purified Crosslinked APC, we manufacture a variety of APC conjugates for different applications. We also offer SMCC-activated APC for simplified conjugation to antibodies and other sulfhydryl-containing proteins of interest. Custom conjugations to APC are also available.

Phycobiliprotein

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Using our highly purified Agilent Phycobiliproteins (formerly ProZyme), we manufacture a variety of APC conjugates for tetramer, FRET Screening, flow cytometry and bead-based assays. Conjugates are purified to remove unreacted components in order to improve sensitivity and reduce non-specific binding. We also offer kits and SMCC-activated phycobiliproteins for simplified conjugation to your antibody or other thiol-containing protein of interest.
Products include Allophycocyanin APC (Spirulina platensis) cross-linked and conjugated to streptavidin and available in two versions. Streptavidin-Allophycocyanin, PJ27S, is specifically designed for use in tetramer and multimer applications. Streptavidin-Allophycocyanin, PJ25S, is a larger conjugate than PJ27S and is better suited for fluorescence resonance energy transfer (FRET) assays.
Also available, APC (Spirulina platensis) cross-linked and conjugated to ANTI-FLAG® M2 monoclonal antibody, and APC cross-linked and conjugated to Goat Anti-GST (Type 1) antibody.

APC Conjugates

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Agilent Streptavidin, Phycobiliproteins and Conjugates

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