Small RNA ladder for sizing
The Small RNA ladder is separated on the Agilent 5200 Fragment Analyzer system with the Small RNA kit. The ladder consists of seven RNA fragments, allowing the sizing of small RNA and miRNA samples.
DNF-470-0275
Small RNA Kits
RUO
The Agilent Small RNA kit focuses on quality control of microRNA (miRNA) and small RNA. Analysis of small RNA is essential for downstream applications such as small RNA next-generation sequencing (NGS) libraries. The Agilent Small RNA ladder includes seven fragments ranging from 15 to 200 nt, providing quantification and sizing of small RNA and miRNA. Focusing on a narrow range of 200 nt magnifies the individual areas of miRNA (10 to 40 nt) and small RNA (40 to 200 nt), allowing for high separation resolution and detailed analysis of each region.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- High sensitivity - Detect concentration as low as 25 pg/µL of purified small RNA
- Short analysis time - Obtain results for 12, 48, or 96 samples in as little as 24 minutes
- Wide sizing range - Analyze small RNA and miRNA samples of 15 to 200 nt through the optimized Small RNA ladder with seven RNA fragments
- Automated, high-resolution analysis - Obtain the percentage and quantity of miRNA as well as the quantity of small RNA in a sample using Agilent ProSize data analysis software
- Minimal sample consumption - Use only 2 µL of precious RNA sample, reducing sample loss during quality control steps
How It Works
Poly(A) tails of IVT mRNA analyzed with the Small RNA kit
The size and quality of the poly(A) tail is a critical part of in vitro transcription (IVT) mRNA workflows because the length of a poly(A) tail affects mRNA stability and influences translation efficiency. Agilent Fragment Analyzer systems with the Small RNA kit is a cost-effective, efficient, and reliable way to estimate the length of poly(A) sequences.
Quantification of small RNA and miRNA
Knowing the size and quantity of small RNA that is present in a sample is crucial for downstream applications such as small RNA NGS, miRNA microarrays, and qRT-PCR. The Small RNA kit is focused on a smaller sizing range and can distinguish between the miRNA region (10 to 40 nt) and the small RNA region (10 to 200 nt). This kit sizes and quantifies the small RNA present in an RNA sample and determines the concentration and percentage of miRNA using ProSize data analysis software.
