Femto Pulse system
Femto Pulse System
The ability to separate high molecular weight DNA up to 165 kb and detect nucleic acids down to 50 fg/µL input concentration make the Femto Pulse system ideal for long-read NGS QC, gDNA, small RNA, or cfDNA analysis from low concentration samples.
- Femto Pulse Systems
Product Details
- Large Fragment Resolution - Separate high molecular weight DNA smears and fragments through 165 kb in about 1.5 hours.
- Femtogram Level Sensitivity - Achieve 10 times higher sensitivity for nucleic acid smears and up to 100 times higher for nucleic acid fragments.
- Simple Sample Preparation - Minimize sample degradation with fewer pipetting steps and stable reagents.
- Powerful Analysis Software - One intuitive software program automates analysis of diverse sample types.
- Conserve Precious Sample - Unparalleled sensitivity allows you to use less precious sample and achieve superior detection.
- Make Workflows More Efficient - Eliminate pulsed-field gel electrophoresis from long-read NGS library prepartion and BAC analysis.
- Desktop PC supplied with a comprehensive 3-year warranty through HP
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- Key Literature
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Visualizing DNA for Long-Read Sequencing by Moles, Not Mass
Visualization of the sample by moles using the Femto Pulse system can provide a clearer assessment of the length distribution, and better predict sequencing results.
- Application Notes
- English
- 07 Apr 2024
- 645.41 KB
Bacterial Artificial Chromosome Analysis on the Agilent Femto Pulse System
The Agilent Femto Pulse system was successfully employed as a screening tool for BAC quality by providing faster visualization and sizing of digested BAC DNA fragments.
- Application Notes
- English
- 08 May 2023
- 610.75 KB
Comparison of Constant- and Pulsed-Field Electrophoresis Technologies for Analysis of High Molecular Weight and Large DNA Fragments
This application note demonstrates the use of the Agilent Fragment Analyzer and Femto Pulse systems for analysis of high-molecular-weight and large DNA fragments.
- Application Notes
- English
- 22 Jun 2023
- 540.88 KB
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- User Manuals
Ferris Jung
Research Technician, EMBL Genomics Core Facility
The Femto Pulse helps us to reliably evaluate the quality of genomic DNA in an easy and straight forward way. Since the quality of the input material is the bottle neck of NGS applications, it gives us the possibility to optimize our protocols effectively, especially for applications like Oxford Nanopore.
View MoreDr. Bruno Huettel
Max Planck-Genome-centre Cologne
The Femto Pulse definitely filled an existing gap in quality control (QC) for RNA and DNA. Especially from chromatin immune precipitated (ChIP) samples it was very difficult to draw some conclusions in QC with previous QC methods because of the very low amounts (low picogram range) usually provided by the user/customers. The pulse field function of the Femto Pulse makes it a very useful tool to judge on the quality of input samples and libraries for Nanopore and PacBio sequencing.
View MorePeter Schweitzer
Director, Genomics Facility, Cornell University
Anybody who’s run PFGE will appreciate the ease-of-use and speed of the Femto Pulse for analyzing high molecular weight DNA. It’s become a critical tool for preparing samples for the long read platforms, both PacBio and Oxford Nanopore, ensuring high quality/high yield on those instruments. Also, with the boundaries of technology being pushed to lower-and-lower sample input, especially for RNA/single cell technologies, the extremely high sensitivity of the Femto Pulse allows us to assess the quality of samples at concentrations that hasn’t been possible before.
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