FP-1002-0275
Genomic DNA 165 kb Kit
Long-read sequencing library preparations involve multiple gDNA and library quality control steps. Compared to standard 16-hour pulsed-field gel electrophoresis (PFGE) protocols, the Genomic DNA 165 kb kit delivers results in just 1.5 hours, significantly reducing the time and effort required in the long-read sequencing library workflow.
- Femto Pulse System DNA Analysis Kits
Product Details
- Reliable sizing — Ensure high accuracy and precision on the sizing of HMW gDNA samples ranging from 1,300 bp to 165 kb
- Fast time to results — Optimized pulsed-field separation methods reduce analysis time and eliminate the need for overnight PFGE separations
- Quality metrics — Quickly assess the integrity of HMW gDNA with the Genomic Quality Number (GQN).
- Ultrasensitive detection — Detect input concentrations as low as 5 pg/µL
- Low sample volume — Require only 2 µL of sample, conserving material for downstream analysis
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- Key Literature
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Quality Control in the PacBio HiFi Whole Genome Sequencing Library
Accurate and reliable next-generation sequencing (NGS) results rely on starting with high-quality samples.
- Application Notes
- English
- 02 Jul 2025
- 1.77 MB
Visualizing DNA for Long-Read Sequencing by Moles, Not Mass
Visualization of the sample by moles using the Femto Pulse system can provide a clearer assessment of the length distribution, and better predict sequencing results.
- Application Notes
- English
- 07 Apr 2024
- 645.41 KB
Comparison of Constant- and Pulsed-Field Electrophoresis Technologies for Analysis of High Molecular Weight and Large DNA Fragments
This application note demonstrates the use of the Agilent Fragment Analyzer and Femto Pulse systems for analysis of high-molecular-weight and large DNA fragments.
- Application Notes
- English
- 22 Jun 2023
- 540.88 KB
- Application Notes
- Brochures
- Case Studies
- Data Sheets
- User Manuals
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Agilent Genomic DNA 165 kb Kit Quick Guide for the Femto Pulse System
This Quick Guide lists the necessary steps to analyze HMW, low concentration DNA smears and/or fragments from 1.3-165 kb with the Agilent Genomic DNA 165 kb kit and the Agilent Femto Pulse system.
- User Manuals
- English
- 02 Jun 2025
- 386.94 KB
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