FP-1002-0275
Genomic DNA 165 kb Kit
RUO
The Genomic DNA 165 kb kit is used in the assessment of high molecular weight (HMW) genomic DNA quality control required for long-read NGS libraries. This kit is designed to run with an optimized pulsed-field separation method enabling accurate sizing of HMW gDNA through 165 kb.
Long-read NGS library preparations require several gDNA quality control steps. Compared to standard 16-hour PFGE protocols, the Genomic DNA 165 kb kit produces results in as little as 1.5 hours. Replacing traditional PFGE with the Genomic DNA 165 kb kit eliminates days from the tedious library workflow.
Long-read NGS library preparations require several gDNA quality control steps. Compared to standard 16-hour PFGE protocols, the Genomic DNA 165 kb kit produces results in as little as 1.5 hours. Replacing traditional PFGE with the Genomic DNA 165 kb kit eliminates days from the tedious library workflow.
For Research Use Only. Not for use in diagnostic procedures.
- Femto Pulse System DNA Analysis Kits
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Product Details
Features
- Reliable Sizing - Accurate and precise sizing of HMW gDNA samples ranging from 1,300 bp to 165 kb
- Reduced Time to Results - Optimized pulsed-field separation methods decrease QC analysis time and eliminates the need for overnight PFGE separations
- Quality Metrics - Genomic Quality Number (GQN) quickly scores the integrity of HMW gDNA
- Ultra-Sensitive Detection - Input concentrations as low as 5 pg/µL
- Low Sample Volume - Only 2 µL required, conserving sample for downstream analysis
Specifications
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| Quantitative Precision |
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How It Works
Literature
- Key Literature
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Visualizing DNA for Long-Read Sequencing by Moles, Not Mass
Visualization of the sample by moles using the Femto Pulse system can provide a clearer assessment of the length distribution, and better predict sequencing results.
- Application Notes
- English
- 07 Apr 2024
- 645.41 KB
Comparison of Constant- and Pulsed-Field Electrophoresis Technologies for Analysis of High Molecular Weight and Large DNA Fragments
This application note demonstrates the use of the Agilent Fragment Analyzer and Femto Pulse systems for analysis of high-molecular-weight and large DNA fragments.
- Application Notes
- English
- 22 Jun 2023
- 540.88 KB
Build Consistency into Your Lab’s Workflow with the Agilent Automated Electrophoresis portfolio.
The automated electrophoresis instrument portfolio includes systems for objective assessment of sample integrity, concentration, and fragment size. Download this eBrochure for an interactive experience.
- Brochures
- English
- 03 Jan 2023
- 10.96 MB
- Application Notes
- Brochures
- Case Studies
- Data Sheets
Support
- User Manuals
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Agilent Genomic DNA 165 kb Kit Quick Guide for the Femto Pulse System
This Quick Guide lists the necessary steps to analyze HMW, low concentration DNA smears and/or fragments from 1.3-165 kb with the Agilent Genomic DNA 165 kb kit and the Agilent Femto Pulse system.
- User Manuals
- English
- 04 Jul 2022
- 292.10 KB
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