PfuUltra II DNA Polymerases & PCR Master Mixes - Details & Specifications
The engineered PfuUltra II Fusion HS DNA Polymerase provides the world's most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.
Industry Leading Fidelity
Our PfuUltra II Fusion HS DNA polymerase is the industry standard for ultra-high fidelity PCR. Our published and peer reviewed data demonstrates that the PfuUltra II Fusion HS DNA polymerase exhibits an accuracy 3-fold higher than any other high fidelity enzyme, including Phusion and iProof DNA polymerases, and 20-fold higher than Taq DNA polymerase, making it the most accurate enzyme available.
PfuUltra II Fusion HS DNA Polymerase, the Highest Fidelity PCR Enzyme
The PfuUltra II Fusion HS DNA Polymerase exhibits accuracy 3-fold higher than the Phusion/iProof DNA polymerases and 20-fold higher than Taq DNA Polymerase. The fidelity of each listed enzyme was measured using our validated and referenced fidelity assay (1) (Accuracy is equal to 1/error rate).
In an effort to increase processivity, we fused our PfuUltra DNA polymerase to a proprietary double-stranded DNA binding protein to increase template binding affinity. This domain serves to better anchor the DNA polymerase, preventing early dissociation from the DNA template. We have improved the processivity of our PfuUltra II Fusion HS DNA Polymerase 12-fold over that of Pfu DNA polymerase. Our PfuUltra II enzyme is also shown to be >5-fold more processive than other commercially available fusion-based enzymes. This improved processivity allows the incorporation of more nucleotides per binding event, which enhances PCR yield and shortens the required extension time, thereby saving you time and improving template integrity by minimizing exposure to extreme cycling temperatures.
Longer and More Accurate Amplicons
The PfuUltra II Fusion HS DNA Polymerase amplifies the widest variety of template sizes compared to other proofreading DNA polymerases. Because it requires short cycling times, genomic templates up to 19 kb in length can be amplified in a fraction of the time. In contrast, other commercial fusions exhibit limited target-length capability. Faster cycling provides you with quicker time-to results, higher throughput, and improves template integrity by minimizing exposure to extreme PCR temperatures.
PfuUltra II Fusion HS DNA Polymerase Amplifies a Wide Range of Targets
The PfuUltra II Fusion HS DNA Polymerase amplifies a broad range of genomic DNA targets up to 19 kb in length. In contrast, other commercial enzymes exhibit limited target-length capability.
Faster Time to Results
The use of shorter extension times (15 seconds/kb for <10 kb targets) means that a 19 kb genomic fragment can be amplified in 5 hours (same day analysis), instead of >19 hours (next day analysis) which is required for non-fusion archaeal DNA polymerases.
PfuUltra II Fusion HS DNA Polymerase is a great choice for your cloning applications. With the highest accuracy of any polymerase, specificity due to its hotstart ability, the speed to complete your amplification quickly, and a general robustness, PfuUltra II provides excellent results for all your cloning needs. Amplification with PfuUltra II will result in blunt ends. For cloning with blunt ended amplicons, see our StrataClone products.
Greatest Success Rate with our PfuUltra II Fusion HS DNA Polymerase
A random set of 94 clones from a human cDNA library was amplified from bacterial colonies. The amplicon sizes varied between 1 and 3 kb. The PfuUltra II Fusion HS DNA Polymerase amplified 91 of 94 randomly selected clones (97%) with high yields and a fast cycling time.
For Research Use Only. Not for use in diagnostic procedures