MiracleHyb High-Performance Hybridization Solution - Details & Specifications

The MiracleHyb High-Performance Hybridization Solution was developed for use in Northern analysis when sensitive detection of low-abundance messages is a priority. In typical Northern applications, high complexity RNA samples are size-fractionated by agarose gel electrophoresis, the resolved RNAs are transferred by blotting to a solid support.

The immobilized RNAs may then be interrogated with one or more gene-specific probes. MiracleHyb is useful for hybridization experiments to detect both low- and high-abundance RNAs. When detecting low-abundance transcripts or when high signal intensity is required, a 24-hour hybridization is performed to achieve maximum sensitivity without increasing background levels. When studying high-abundance genes, or when quick results are important, hybridization time may be reduced to 1-2 hours.

For Research Use Only. Not for use in diagnostic procedures