HybriZAP 2.1 Two-Hybrid System - Details & Specifications

The Yeast DNA Isolation System, an innovative system for rapidly retrieving plasmid DNA from positive yeast clones identified from GAL4 two-hybrid system screenings, is designed specifically for the yeast host strains, YRG-2 and HF7c. The method uses a single lysis buffer combined with a rapid freeze–thaw cycle, followed by a heat step. The amount of plasmid DNA retrieved from a single yeast colony is sufficient to transform XL1-Blue supercompetent cells. The entire process, from picking the colony of interest to transformation, takes less than 15 minutes. Yields of 10–200 transformant colonies are typically achieved when using the Yeast DNA Isolation System.

For Research Use Only. Not for use in diagnostic procedures