FLAG Western Detection Kit - Details & Specifications

Epitope tagging technology is a powerful method for the analysis of protein structure and function both in vivo and in vitro, and the FLAG (DYKDDDDK) peptide (from Sigma Aldrich – see licensing information included in the kit) is one of the most widely used epitope tags in the field of molecular biology, cell biology, and genetics for protein labeling. The western detection kit includes an anti-FLAG M2 antibody that can detect recombinant fusion proteins with the FLAG epitope located at either the N- or C-terminal, as well as recombinant fusion proteins with the FLAG epitope located internally. The anti-FLAG M2 antibody can detect as little as 5 ng of FLAG epitope-tagged recombinant fusion protein. The anti-FLAG M2 antibody is highly specific, with low cross-reactivity with prokaryotic and eukaryotic proteins.

The FLAG western detection kit utilizes the anti-FLAG M2 antibody as the primary antibody and an alkaline phosphatase (AP)-conjugated goat anti-mouse antibody as the secondary antibody. The signal is detected by a color development solution containing nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The FLAG western detection kit can be used to monitor expression of FLAG-tagged proteins in prokaryotic and eukaryotic cells, to follow FLAG epitope-tagged protein during protein purification steps, and to identify proteins following immunoprecipitation.

For Research Use Only. Not for use in diagnostic procedures