EXL DNA Polymerase - Details & Specifications

EXL DNA polymerase provides superior performance in amplifying complex targets of lengths greater than 20 kb and provides higher fidelity than Taq DNA polymerase and Taq-based blends. EXL DNA polymerase features a special DNA polymerase composition that has been optimized specifically for extremely long targets. Our EXL DNA polymerase is provided with an optimized buffer, DMSO and a stabilizing solution to facilitate extreme reaction conditions needed for very long targets. EXL DNA polymerase provides robust yield, specificity, and reliable amplification of extra long targets. As a result, the EXL DNA polymerase can be used to successfully amplify genomic targets up to 37 kb and vector targets up to 48 kb, while maintaining a lower error rate than other DNA polymerase mixtures (Figure 1).

A key component of EXL DNA polymerase is the ArchaeMaxx polymerase-enhancing factor. The ArchaeMaxx factor eliminates a PCR inhibitor and promotes shorter extension times, higher yield, and greater target length capabilities. The ArchaeMaxx factor improves the yield of products by overcoming dUTP poisoning, which is caused by dUTP accumulation during PCR through dCTP deamination. Once incorporated, dU-containing DNA inhibits Pfu and most archaeal proofreading DNA polymerases, such as Vent and Deep Vent DNA polymerases, limiting their efficiency. The ArchaeMaxx factor functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate, resulting in improved overall PCR performance.

For Research Use Only. Not for use in diagnostic procedures