Download AGeNT Tool
The Agilent Genomics NextGen tool kit (AGeNT) is a Java-based software module that processes the read sequences from targeted high-throughput sequencing data generated by sequencing Agilent SureSelect and HaloPlex libraries. The trimmer utility of the AGeNT module processes the read sequences to identify and remove the adaptor sequences and extracts dual molecular barcodes (for SureSelect XT HS2). The LocatIt utility of the AGeNT module processes the molecular barcode (MBC) information from HaloPlexHS, SureSelect XT HS, and SureSelect XT HS2 Illumina sequencing runs with options to either mark or merge duplicate reads and output in BAM file format.
DISCLAIMER: The Agilent Genomics NextGen tool kit (AGeNT) software module has been designed to provide the adaptor trimming and duplicate read removal using the Molecular barcode (MBC) information from HaloPlexHS, SureSelect XT HS, and SureSelect XT HS2 Illumina sequencing runs in a flexible command-line interface for integration into your bio-informatics pipeline. AGeNT is explicitly designed and fine-tuned for customers with established in-house bio-informatics experts with the capability to build, integrate, maintain, and Troubleshoot internal analysis pipelines. Moreover, the module is designed specifically for customers with sufficient computing infrastructure and IT support to troubleshoot all issues unrelated to the execution of the AGeNT algorithms. Agilent recommends that users without thorough bio-informatics expertise use either Agilent SureCall or Alissa Align & Call software Instead.
For Research Use Only. Not for use in diagnostic procedures.