Figure 1A. Low Copy Discrimination – Amplification plot for a 2-fold dilution of linearized plasmid run on a StepOnePlus real-time PCR system. Brilliant III Ultra-Fast QPCR master mix exhibits precise detection of 2-fold differences from 1536 copies down to 3 copy equivalents.
The new Brilliant III Ultra-Fast QPCR Master Mixes deliver QPCR results in around 40 minutes on the StepOnePlus Real-Time PCR system. The enhanced sensitivity, specificity and reproducibility within an assay and across multiple assays from high to very low copy number templates makes Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes your preferred choice for Real-Time PCR analysis.
Our next generation Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mix Kits offer significantly reduced cycling time and superior sensitivity without compromising quantification and reproducibility. The Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes are designed to provide the shortest cycling times on fast cycling real-time PCR instruments (Figure 1A & 1B).
Highly efficient one-step QRT-PCR is performed with our Brilliant III Ultra-Fast QRT-PCR reagents using a Moloney-based RT for 1st strand synthesis with optimal performance at a synthesis temperature of 50°C.
AffinityScript QPCR cDNA Synthesis Kit can be used for 1st strand cDNA synthesis in a 2-step providing flexibility across a wide range of temperatures. Novel hotstart Taq DNA polymerase combined with AffinityScript RT, minimizes the potential for primer-dimer formation or other non-specific PCR products and delivers the most reproducible results.
Figure 1B. Standard curve plot for a 2-fold dilution of linearized plasmid run on a StepOnePlus real-time PCR system. The novel hot start technology of Brilliant III Ultra-Fast QPCR reagents decreases primer-dimer formation and amplification of unwanted side reactions resulting in superior sensitivity of detection down to very low target concentrations. Efficiency = 95.7%, RSq. = 0.992.
For Research Use Only. Not for use in diagnostic procedures