Brilliant II SYBR Green QPCR & QRT-PCR Master Mixes - Details & Specifications

The Brilliant II SYBR Green QPCR master mix includes the components necessary to carry out QPCR amplifications with SYBR Green detection. The improved Brilliant II formulation yields higher levels of final fluorescence and earlier Ct values for many genomic DNA and cDNA targets and the master mix format is ideal for high-throughput applications. The Brilliant II master mix has been optimized for a faster two-step cycling protocol that is 25% shorter than the protocol used with the original Brilliant SYBR Green QPCR master mix. The Brilliant II master mix is also ideal for quantification of cDNA in a 2-step QRT-PCR reaction when combined with the AffinityScript QPCR cDNA Synthesis Kit.

The Brilliant II SYBR Green QPCR master mix has been optimized for maximum performance on the Stratagene Mx3000P and Mx3005P real-time PCR systems and Stratagene Mx4000 multiplex quantitative PCR system, as well as on the ABI 7900HT real-time PCR instrument. In addition, excellent results have been observed using most other QPCR platforms.

The Brilliant II SYBR Green QPCR master mix includes SureStart Taq DNA polymerase, a modified version of Taq2000 DNA polymerase with hot start capability. SureStart Taq DNA polymerase improves PCR amplification reactions by decreasing background from nonspecific amplification and increasing amplification of desired products. Using SureStart Taq, hot start is easily incorporated into PCR protocols already optimized with Taq DNA polymerase, with little modification of cycling parameters or reaction conditions. A passive reference dye (an optional reaction component) is provided in a separate tube, making the master mix adaptable for many real-time QPCR platforms.

For Research Use Only. Not for use in diagnostic procedures