Adrenal Gland Total RNA - Details & Specifications

RNA quality is assessed visually by observing distinct 28S and 18S ribosomal bands on a non-denaturing gel. Purity of the RNA is assessed by spectrophotometry (A260/A280 > 1.8). RNA is further shown to be free of contaminating RNases by incubation in a suitable buffer at 37ºC.

Total RNA and Poly(A)+ RNA products are subjected to stringent quality assurance functional testing, including Northern analysis and RT-PCR.

Products were isolated using a modified guanidinium thiocyanate method. Human tissue was obtained using protocols approved by an Institutional Review Board. Total RNA is enriched for mRNA by two successive rounds of Oligo(dT)-cellulose selection to produce our Poly(A)+ RNA.

• Northern analysis
• cDNA synthesis
• QRT-PCR
• In vitro translation
• Ribonuclease protection assay
• S1 nuclease analysis
• Microarray gene expression analysis

For Research Use Only. Not for use in diagnostic procedures