Agilent Bio SEC-3

Agilent Bio SEC-3

Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle. Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations.

View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.

Biomolecule Separations

특징

Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
Sharper peaks, higher resolution, and better protein recovery
Faster separations than large-particle SEC columns
Compatibility with most aqueous buffers
Excellent stability in high-salt and low-salt conditions

툴

Use the new LC Column and Sample Prep Navigator to find the best Agilent column and sample prep products for your application.
Try it now at www.agilent.com/chem/navigator

Search for LC Application Notes and Technical Overviews using our new Application Finder! Narrow down your search by applying and combining filters for system, column, market and compound. Select the publication, get a quick preview, and then open and download it.

Agilent offers a complete portfolio of supplies for all other major instrument brands, including Bruker, PerkinElmer, Waters, Thermo/Dionex and Shimadzu. These parts are manufactured to perform seamlessly with any of these instruments. These parts are backed by our same commitment to quality and our money back guarantee, as well as our worldwide technical support team, available to answer any question or concerns you may have.

Our Syringe Filter Online Selection Guide makes it fast and easy to choose the best syringe filter for your application.
Try it now at: www.agilent.com/chem/SelectFilters

Unsure which vials and closures are best suited to your application?
Quickly and easily find the perfect match using our interactive tool: www.agilent.com/chem/SelectVials

추가 정보

TIPS & TOOLS

Deactivated/silanized vials have inert surfaces that will not interact with metals, biologicals or proteins, and will not cause pH shifts. Avoid standard polypropylene vials for biological or light-sensitive compounds.

SEC Column Methods

Pore Size	Particle Size	MW Range	pH Range	Max Pressure	Flow Rate
100Å	3 µm	100-100,000	2-8.5	240 bar, 3500 psi	0.1-1.25 mL/min (7.8 mm id)
					0.1-0.4 mL/min (4.6 mm id)
150Å	3 µm	500-150,000	2-8.5	240 bar, 3500 psi	0.1-1.25 mL/min (7.8 mm id)
					0.1-0.4 mL/min (4.6 mm id)
300Å	3 µm	5,000-1,250,000	2-8.5	240 bar, 3500 psi	0.1-1.25 mL/min (7.8 mm id)
					0.1-0.4 mL/min (4.6 mm id)

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