储存缓冲液 |
- 50 mM Tris-HCl (pH 7.5), 50 % glycerol, 1 mM EDTA, 10 mM DTT, 0.1 % Tween 20, 0.1 % Igepal
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单位定义 |
- One unit will ligate to completion 0.5 µg of nicked pBluescript DNA in 15 minutes at 55°C.
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反应条件 |
- 4 U of Pfu DNA ligase enzyme, in 20 mM Tris-HCl (pH 7.5), 20 mM KCl, 10 mM MgCl2, 0.1 % Igepal, 0.01 mM rATP, 1 mM DTT
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浓度 |
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连接酶类型 |
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