R-Phycoerythrin (R-PE) Conjugation Kit
R-Phycoerythrin (R-PE) Conjugation Kit (formerly ProZyme) contains everything you need to conjugate up to 1 mg of your antibody to RPE. The kit method employs reduction of disulfides in your antibody to produce free sulfhydryl groups which are then reacted with maleimide groups on SMCC-RPE. Complete step-by-step protocols are included and allow small scale conjugations down to 50 µg of antibody. The kit is suitable for conjugation of other sulfhydryl-containing proteins as well. It utilizes a widely used and reliable conjugation chemistry and the highest-purity RPE available. Conjugates can be ready in as little as two hours.