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This procedure reflects best practices for replacing the growth medium with assay medium for adherent cells grown in XF96 Cell Culture Microplates prior to being assayed using an Agilent Seahorse XFe96/XF96 Analyzer.
- Publication Part Number: 5994-7157EN
- Created: 21 Aug 2023
- 1 MB
This application note describes the use of human primary hepatocytes in the context of the Agilent Seahorse XF Mito Tox assay, including assay optimization,
- Publication Part Number: 5994-6476EN
- Created: 08 Aug 2023
- 2 MB
Product portfolio brochure includes information about MitoXpress Xtra and pH Xtra, assays for measuring metabolism on fluorescence plate readers
- Publication Part Number: 5994-0683EN
- Created: 13 Mar 2023
- 1 MB
This application note introduces a workflow that uses the XF Real-Time ATP rate assay data for identifying metabolic modulator and drug targets.
- Publication Part Number: 5994-5651EN
- Created: 27 Jan 2023
- 2 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|
This guide explains how to use the VWorks software to Set up devices, Write protocols, Run protocols, Troubleshoot protocols, and Operate devices using VWorks software 14.4.
- Publication Part Number: D0004378
- Created: 21 Oct 2022
- 25 MB
This application note demonstrates the feasibility and use of combining multiple cell-based in vitro assays from Agilent that use diverse analytical technologies to investigate the cardiac liability of drugs.
- Publication Part Number: 5994-5319EN
- Created: 12 Sep 2022
- 1 MB
Learn about a customized workflow to standardize the identification/investigation of mitochondrial toxicity using Agilent Seahorse XF technology.
- Publication Part Number: 5994-4778EN
- Created: 27 Apr 2022
- 3 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|
User guide for the pH Xtra Glycolysis Assay kit. Includes workflow, plate reader-, storage conditions and signal optimization instructions.
- Publication Part Number: 5991-9329EN
- Created: 01 May 2021
- 517 KB
Consumables workflow ordering guide for sialic acid quantitation of biotherapeutic glycoproteins using AdvanceBio Total Sialic Acid Quantitiation Kits.
- Publication Part Number: 5994-4383EN
- Created: 06 Jan 2021
- 346 KB
| Spanish (Spain) | Complete (PDF) |
|---|---|
| French (France) | Complete (PDF) |
| Chinese (Simplified) (China) | Complete (PDF) |
| Italian (Italy) | Complete (PDF) |
| German (Germany) | Complete (PDF) |
| Korean (Korea) | Complete (PDF) |
| Japanese (Japan) | Complete (PDF) |
Two workflows using MitoXpress Xtra and pH-Xtra probes to investigate cell metabolism in intact RAFT 3D collagen structures on fluorescent plate readers.
- Publication Part Number: 5994-2195EN
- Created: 12 Aug 2020
- 876 KB
This Quick Start Guide provides all you need to know from plate reader setup and signal optimization, to sample and compound optimizations, as well as routine assay runs and data analysis for the MitoXpres Xtra Oxygen Consumption Assay.
- Publication Part Number: 5994-1822EN
- Created: 05 May 2020
- 1 MB
The Agilent XF Substrate Oxidation Stress Tests facilitate investigation of mitochondrial substrates that are required for cellular phenotype/function
- Publication Part Number: 5994-1623EN
- Created: 23 Apr 2020
- 2 MB
| Russian (Russia) | Complete (PDF) |
|---|---|
| French (France) | Complete (PDF) |
| Chinese (Simplified) (China) | Complete (PDF) |
| Korean (Korea) | Complete (PDF) |
User guide for the MitoXpress Intra Assay kit. Includes workflow, plate reader-, storage conditions and signal optimization instructions.
- Publication Part Number: 5991-9327EN
- Created: 22 Oct 2019
- 1 MB
| English (United States) | Complete (PDF) |
|---|
User guide for the MitoXpress Xtra Oxygen Consumption Assay kit. Includes workflow, plate reader-, storage conditions and signal optimization instructions.
- Publication Part Number: 5991-9328EN
- Created: 22 Oct 2019
- 1 MB
A demonstration of the performance of the Agilent MitoXpress Xtra oxygen consumption assay for identifying drug induced mitocondrial toxicity.
- Publication Part Number: 5994-1216EN
- Created: 30 Aug 2019
- 872 KB
User manual for Seeding Cells in XFe96 Cell Culture Microplates
- Publication Part Number: 5994-0238EN
- Created: 11 Apr 2019
- 646 KB
The Seahorse XF neutrophil activation assay via measuring oxygen consumption rate provides temporal resolution of neutrophil activation not possible by other assays.
- Publication Part Number: 5991-9118EN
- Created: 12 Mar 2018
- 1 MB
Agilent history and timeline
In this TekTalk we talk about Enzyme-linked immunosorbent assay (ELISA), an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process.
In this TekTalk we describe automated imaging and analysis methods for two common genotoxicity assay formats-the comet assay and gH2AX assay.