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Cell migration is a fundamental process in the development and maintenance of multicellular organisms. It is essential for tissue formation during embryonic development, wound healing, and immune responses.
In this TekTalk we decribe the most popular in vitro method to monitor immune cell-mediated cytotoxicity on target cells is the cell-mediated cytotoxicity (CMC) assay, in which immune effector cells and target cells are added to a microplate as a...
An optimized Agilent XF 3D tissue workflow that allows for detection and quantitative assessment of energy metabolism in tissue materials is presented.
- Publication Part Number: 5994-8309EN
- Created: 30 Apr 2026
- 3 MB
| Japanese (Japan) | Complete (PDF) |
|---|
Robust microplate-based approach for quantifying induced activation of T cells utilizing automated imaging, luminescence, and ELISA.
- Publication Part Number: 5994-8474EN
- Created: 04 Aug 2025
- 698 KB
User manual for the Seahorse XF 3D Mito Stress Test Assay.
- Publication Part Number: 5994-8177EN
- Created: 22 May 2025
- 579 KB
Demonstration of the wide range of metabolic rates that can be quantified on the Agilent Seahorse XF Flex analyzer through the use of different Seahorse XF microplates and in vitro differentiation of adipocytes.
- Publication Part Number: 5594-8321EN
- Created: 22 May 2025
- 2 MB
This application note uses the Fragment Analyzer and HS RNA kit and provides an example of the calculation of encapsulation efficiency by quantifying IVT mRNAs before and after LNP disruption.
- Publication Part Number: 5994-8036EN
- Created: 20 Feb 2025
- 494 KB
This document provides guidelines and a detailed protocol on how to use the Agilent Mito-rOCR Assay Kit.
- Publication Part Number: 5994-7821EN
- Created: 30 Sep 2024
- 606 KB
Agilent CrossLab services for your molecular biology applications.
- Publication Part Number: 5994-1585EN
- Created: 04 Mar 2024
- 2 MB
This guide explains how to set up the VWorks 14.4 software, create labware definitions and liquid classes, create a labware inventory database, create an experiments database, and how to migrate protocols from earlier versions.
- Publication Part Number: D0004377
- Created: 28 Nov 2023
- 7 MB
This procedure reflects best practices for replacing the growth medium with assay medium for adherent cells grown in XF96 Cell Culture Microplates prior to being assayed using an Agilent Seahorse XFe96/XF96 Analyzer.
- Publication Part Number: 5994-7157EN
- Created: 21 Aug 2023
- 1 MB
User manual for Washing Cells in XF96 Cell Culture Microplates
- Publication Part Number: 5994-0240EN
- Created: 21 Aug 2023
- 1 MB
| English (United States) | Complete (PDF) |
|---|
This application note describes the use of human primary hepatocytes in the context of the Agilent Seahorse XF Mito Tox assay, including assay optimization,
- Publication Part Number: 5994-6476EN
- Created: 08 Aug 2023
- 2 MB
Product portfolio brochure includes information about MitoXpress Xtra and pH Xtra, assays for measuring metabolism on fluorescence plate readers
- Publication Part Number: 5994-0683EN
- Created: 13 Mar 2023
- 1 MB
This application note introduces a workflow that uses the XF Real-Time ATP rate assay data for identifying metabolic modulator and drug targets.
- Publication Part Number: 5994-5651EN
- Created: 27 Jan 2023
- 2 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|
This guide explains how to use the VWorks software to Set up devices, Write protocols, Run protocols, Troubleshoot protocols, and Operate devices using VWorks software 14.4.
- Publication Part Number: D0004378
- Created: 21 Oct 2022
- 25 MB
This application note demonstrates the feasibility and use of combining multiple cell-based in vitro assays from Agilent that use diverse analytical technologies to investigate the cardiac liability of drugs.
- Publication Part Number: 5994-5319EN
- Created: 12 Sep 2022
- 1 MB
Learn about a customized workflow to standardize the identification/investigation of mitochondrial toxicity using Agilent Seahorse XF technology.
- Publication Part Number: 5994-4778EN
- Created: 27 Apr 2022
- 3 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|
User guide for the pH Xtra Glycolysis Assay kit. Includes workflow, plate reader-, storage conditions and signal optimization instructions.
- Publication Part Number: 5991-9329EN
- Created: 01 May 2021
- 517 KB