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This application note describes a methodology to quantitate nucleic acids using an Agilent BioTek microplate spectrophotometer.
3rd party device driver guide
Newsletter
In this issue of TekTalk we show how the Agilent BioTek automated imaging systems enable kinetic live-cell experiments over broad timescales.
Agilent scientists integrated industry leading platforms - BioTek, Acea, Seahorse and Luxcel - with Agilent's mass spectrometry. This unique combination allows scientists to measure cancer morphologically, sub-cellularly and non-invasively.
Newsletter
Here we describe the broad range of microbiology applications enabled by Agilent BioTek automated imagers, plate readers, & purpose-build LP600.
| Authors | Berger E and Geloen A |
|---|---|
| Journal | Adipocyte |
| Publication Date | 01 Dec 2022 |
| Research Area(s) include | Cell Biology, Obesity, Diabetes and Metabolic Disorders |
| Technique | Automated Microscopy and Imaging, Flow Cytometry, xCELLigence Real Time Cell Analysis Technology |
| Analyzer(s) include | BioTek Cytation 3, NovoCyte Flow Cytometer, RTCA E-Plate 96, xCELLigence RTCA SP - Single Plate (96 well) |
| Mode | N/A |
| Cell Line(s) include | 3T3-L1 |
| Cell Type(s) include | Adipocytes, Fibroblast |
| Species | Human |
| Assay / Method | Cell Adhesion & Spreading, Not Specified |
| Cell Seeding Density | 5.0x10^3 cells/well |
Agilent scientists integrated industry leading platforms - BioTek, Acea, Seahorse and Luxcel - with Agilent's mass spectrometry. This unique combination allows scientists to measure cancer morphologically, sub-cellularly and non-invasively.
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
This technical overview describes the materials used in the module and information to assist in determining their compatibility with common chemicals.
This technical overview provides information on programming long equations into the BioTek microplate readers onboard software.
This application note describes the use of the MicroFlo Select dispenser to easily and reliably dispense hot molten agar solutions into 96- and 384-well microplates.
Application Note - Normalization of Agilent Seahorse XF data by in-situ cell counting using a BioTek Cytation 5
| Authors | Zhou X, Zhu S, Li J, Mateus A, Williams C, Gilthorpe J and Backman LJ |
|---|---|
| Journal | J Proteome Res |
| Publication Date | 30 Aug 2024 |
| Research Area(s) include | Cell Physiology Research |
| Technique | Microplate Reader Detection, Seahorse XF Technology |
| Analyzer(s) include | BioTek Synergy HTX, Seahorse XF 96 well (XF Pro/XFe96/XF96) |
| Mode | N/A |
| Cell Line(s) include | Myoblasts |
| Cell Type(s) include | Myoblasts |
| Species | Not Specified |
| Assay / Method | Cell Mitochondrial Stress Test |
| Cell Seeding Density | 6.0x10^4 cells/well |
| Plate Coating | Poly-D-lysine (50 µg/ml) |
| Authors | Chatterjee S, Leach-Mehrwald M, Huang CK, Xiao K, Fuchs M, Otto M, Lu D, Dang V, Winkler T, Dunbar CE, Thum T, Bar C |
|---|---|
| Journal | Cell Mol Life Sci |
| Publication Date | 24 Apr 2024 |
| Research Area(s) include | Cardiovascular Research, Cardiovascular Research |
| Technique | Automated Microscopy and Imaging, Seahorse XF Technology, xCELLigence Real Time Cell Analysis Technology |
| Analyzer(s) include | BioTek Cytation 1, Seahorse XF 96 well (XF Pro/XFe96/XF96), xCELLigence RTCA Cardio - Cardiomyocyte Contractility |
| Mode | N/A |
| Cell Line(s) include | hiPSC-CMs |
| Cell Type(s) include | Cardiac cells |
| Species | Human |
| Assay / Method | Cardiac Disease Modeling, Cell Mitochondrial Stress Test |
| Cell Seeding Density | 5.0x10^4 cells/well, Not Specified |
| Plate Coating | Not Specified |
| Authors | Chavez JD, Keller A, Zhou B, Tian R and Bruce JE |
|---|---|
| Journal | Cell Rep |
| Publication Date | 19 Nov 2019 |
| Research Area(s) include | Cancer Research, Cell Biology, Drug Discovery |
| Technique | Automated Microscopy and Imaging, Seahorse XF Technology |
| Analyzer(s) include | BioTek Cytation 5, Seahorse XFe24/XF24 |
| Mode | N/A |
| Cell Line(s) include | HeLa |
| Cell Type(s) include | Cervical cancer cells |
| Species | Human |
| Assay / Method | Cell Mitochondrial Stress Test |
| Cell Seeding Density | 5.0x10^4 cells/well |
| Plate Coating | Not Specified |
| Authors | Hwang J, Park A, Kim C, Yu D, Byun H, Ku M, Yang J, Kim TI, Jeong KS, Kim KY, Lee H, Shin SJ |
|---|---|
| Journal | Anticancer Research |
| Publication Date | 01 Jan 2022 |
| Research Area(s) include | Cancer Biology, Cancer Research |
| Technique | Automated Microscopy and Imaging, Seahorse XF Technology, xCELLigence Real Time Cell Analysis Technology |
| Analyzer(s) include | BioTek Cytation 1, RTCA E-Plate 16, Seahorse XFe24/XF24, xCELLigence RTCA DP - Cell Invasion and Migration (16-well) |
| Mode | N/A |
| Cell Line(s) include | HCT116, SW-480 |
| Cell Type(s) include | Colon cancer cells |
| Species | Human |
| Assay / Method | Cell Energy Phenotype Test, Cell Proliferation, Cytotoxicity/Cell Death |
| Cell Seeding Density | 4.0x10^4 cells/well, 5.0x10^4 cells/well |
| Plate Coating | Not Specified |
| Authors | D Wang, Y Zhang, S Yang, D Zhao, and M Wang |
|---|---|
| Journal | Int J Biol Macromol |
| Publication Date | 15 Mar 2019 |
| Research Area(s) include | Cell Metabolism, Drug Discovery, Immunology Research |
| Technique | Automated Microscopy and Imaging, Plate Reader-Soluble Sensor |
| Analyzer(s) include | BioTek Cytation 5, Plate Reader Metabolic Assays-96 well |
| Mode | N/A |
| Cell Line(s) include | Caco-2 |
| Cell Type(s) include | Colon cancer cells |
| Species | Human |
| Assay / Method | MitoXpress Xtra Oxygen Consumption Assay |
| Cell Seeding Density | Not Specified |
| Plate Coating | Not Specified |
| Authors | Shan H, Chen M, Zhao S, Wei X, Zheng M, Li Y, Lin Q, Jiang Z, Chen Z, Fei C, Li Z, Chen Z and Chen X |
|---|---|
| Journal | Sci Adv |
| Publication Date | 22 Nov 2024 |
| Research Area(s) include | Cancer Biology |
| Technique | Automated Microscopy and Imaging |
| Analyzer(s) include | BioTek Cytation C10 |
| Mode | N/A |
| Authors | Yu X, Dai Y, Ji X, Pu Q, Zhang R, Shi M, Hu N, Jin K, Zhu J and Li J |
|---|---|
| Journal | Virus Res |
| Publication Date | 02 Feb 2026 |
| Research Area(s) include | Virology |
| Technique | Automated Microscopy and Imaging |
| Analyzer(s) include | BioTek Cytation 1 |
| Mode | N/A |
| Authors | Herbers E, Patrikoski M, Wagner A, Jokinen R, Hassinen A, Heinonen S, Miettinen S, Peltoniemi H, Pirinen E and Pietilainen K H |
|---|---|
| Journal | Stem Cells Int |
| Publication Date | 05 Apr 2022 |
| Research Area(s) include | Cell Metabolism |
| Technique | Automated Microscopy and Imaging |
| Analyzer(s) include | BioTek Cytation 5 |
| Mode | N/A |