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This compendium describes quantitative imaging-based methods designed to provide objective evaluation of several variables that contribute to experimental results.
- Publication Part Number: 5994-3007EN
- Created: 01 Feb 2021
- 1 MB
This application note evaluates chemically induced cell stress by thapsigargin, while simultaneously measuring its effects on Gq-mediated cell signaling.
- Publication Part Number: 5994-2587EN
- Created: 01 Feb 2021
- 758 KB
This application note describes the use of the EL406 in conjunction with BioStack and LHC software to semi-automate a melamine ELISA.
- Publication Part Number: 5994-2689EN
- Created: 01 Feb 2021
- 165 KB
This study uses the Cytation 7 cell imager and Gen5 software to quantitate changes in cytokine secretion in PBMCs using the colorimetric ELISpot assay format.
- Publication Part Number: 5994-2398EN
- Created: 01 Feb 2021
- 458 KB
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This application note demonstrates the utility of using the Cytation 3 and genetically encoded probes to detect GPCR signaling products in live cells in real time.
- Publication Part Number: 5994-2525EN
- Created: 01 Feb 2021
- 637 KB
This application note demonstrates an in vitro live cell assay to monitor NETosis activity using differentiated HL-60 cells as a model.
- Publication Part Number: 5994-2572EN
- Created: 01 Feb 2021
- 3 MB
This application note demonstrates the ability to monitor cell migration and invasion using a novel cell imaging multimode reader.
- Publication Part Number: 5994-2751EN
- Created: 01 Feb 2021
- 598 KB
This application note demonstrates the ability of the Cytation 5 in conjunction with Gen5 analysis to calculate HIV infection rates in fixed cells.
- Publication Part Number: 5994-2542EN
- Created: 01 Feb 2021
- 249 KB
This application note demonstrates the optimization and validation of cellular energy flux probes using a variety of Agilent BioTek readers and detection methods.
- Publication Part Number: 5994-2586EN
- Created: 01 Feb 2021
- 617 KB
The Cytation 5, paired with BioSpa automated incubator, provides a fully automated means of quantifying image-based assays of cell proliferation and cell death.
- Publication Part Number: 5994-2593EN
- Created: 01 Feb 2021
- 644 KB
This application note demonstrates the automation of the hemocytometer-based live/dead cell counting process using phase contrast and color brightfield imaging.
- Publication Part Number: 5994-2598EN
- Created: 01 Feb 2021
- 2 MB
This application note describes the use of the LogPhase 600 microbiology reader to provide temperature control, suspension agitation, and monitor bacterial growth.
- Publication Part Number: 5994-2549EN
- Created: 01 Feb 2021
- 656 KB
This application note describes a novel 3D NK cell mediated cytotoxicity (CMC) assay.
- Publication Part Number: 5994-2592EN
- Created: 01 Feb 2021
- 703 KB
This study describes a method for detecting Escherichia coli O157:H7, Listeria monocytogen, and Salmonella enterica Typhimurium in spiked lettuce wash samples.
- Publication Part Number: 5994-2547EN
- Created: 01 Feb 2021
- 547 KB
This study demonstrates the utility of a 3D spheroid cell culture model, used to elucidate the apoptotic potential of two compounds in two different cell lines.
- Publication Part Number: 5994-2591EN
- Created: 01 Feb 2021
- 471 KB
This application note describes an automated microscopy and analysis protocol as an alternative method for AFB scoring.
- Publication Part Number: 5994-2546EN
- Created: 01 Feb 2021
- 462 KB
This study describes a method to incorporate automated, digital widefield phase contrast microscopy to monitor potential apoptotic effects of lead molecules.
- Publication Part Number: 5994-2589EN
- Created: 01 Feb 2021
- 840 KB
This guide offers Step-by-step instructions to maximize the functionality of the MitoXpress Xtra template (AgilentXO2) for BioTek Gen5, including plate reader setup and signal optimization
- Publication Part Number: 5994-2322EN
- Created: 21 Aug 2020
- 2 MB
Learn about Agilent's role in sustainability and sustainable development.
Agilent continues to expand its global cell-analysis footprint with BioTek acquisition